Mireille Consalvey PhD Thesis - University of St Andrews

0 DI-cl
(B) Sampling schedule March 2000
', W-JLI'60W,
-
ll[t, flliýý'1(3ril\, (OI)Crltll-i; ý' bl"A'11m,
Both sites: Day I Arlesford Creek: Day 2 Hythe: Day 2
Time
Cumufative time
(minutes)
06: 30 0
07: 40 70
08: 30 120
09: 25 175
11: 45 315
12: 15 345
13: 05 450
14: 50 500
15: 40 550
1 ,
17: 25 655
18: 10 700
18: 50 740
Primary productivity
Time
Cumulative time
(minutes)
07: 00 0
08: 30 90
08: 501 110
10: 00 180
10: 40 220
12: 45 345
13: 45 405
,
14: 40 460
15: 40 520
17: 25 625
:5 695
.
19: 30 1 750
Time
Cumulative time
(minutes)
07: 30 0
08: 30ý 60
09: 20 110
10: 00 150
10: 55 205
12: 55 325
13: 50 380
14: 50 440
15: 55 505
17: 45 615
18: 40 670
19: 45 ,
Total primary production (ýtg C M-2 h-1) and primary productivity (ýtg C
(ýtg Chl a)-' M-2 h-') (work by Dr RG Perkins) were measured every 2 h.
Sediment cores were selected at random (n = 3) and sub-sampled using a mini-
core (3.14 CM2) (n = 3) (Smith and Underwood 1998). 1 ml of radio-label (37
kBq 14C-sodium bicarbonate) was allowed to diffuse into each mini core for Ih
in the dark. Subsequent to this the mini-cores were incubated for Ih at I of 5
light levels (0 800
- ýtrnol M-2 S-1 ) using neutral density filters on a
halogen light
source. The incubation was terminated by the addition of 5%f. c. gluteraldehyde.
Dark controls (n = 3) were incubated for Ih in the dark to correct
for dark
uptake. In addition anapleurotic uptake was corrected for using dead controls,
which had 5%f, c. gluteraldehyde added prior to the addition of the radio-label.
After termination, the top 2 mm of biofilm was carefully sliced off and freeze-
dried and homogenised. This was then sub-divided into 2 samples. The first set
167
735,