Comparative Parasitology 67(1) 2000 - Peru State College

86 COMPARATIVE PARASITOLOGY, 67(1), JANUARY 2000
goon (18°37'50"N; 90°16'50"W); La Pera Lagoon
(18°16'57"N; 91°56'21"W); Terminos Lagoon, Santa
Gertrudis station (18°26'51"N; 91°48'59"W); Rancho
II station (18°20'30"N; 91°42'30"W); El Viento
station (18°26'01"N; 91°49'48"W). State of Yucatan:
Dzaptiin Cenote (20°51'19"N; 90°14'09"W); Chaamac
Cenote (20°51'53"N; 90°09'18"W); Petentuche
Cenote (21°33'90"N; 88°04'44"W); Dzonot Cervera
Cenote (21°22'36"N; 88°49'59"W); Ojo de Agua ( =
water spring), Celestun Lagoon (20°52'37"N;
90°21'18"W). State of Quintana Roo: Mahahual
(18°58'17"N; 87°57'30"W); Cenote Azul (Bacalar)
(18°38'11"N; 88°24'46"W); Raudales Lagoon
(18°42'27"N; 88°15'22"W); Hondo River (18°17'N;
88°38'W); Valle Hermoso Lagoon (19°10'N;
88°31'W); Rancho Don Milo (18°37'43"N;
88°01'15"W). Guatemala: Champerico River, Department
of Retalhuleu, near the border with El Salvador
(14°20'N; 91°54'W).
Sampling dates and parameters of infection are provided
for each species in the results section. Fishes
were transported alive to the laboratory and dissected
using standard parasitological procedures. Monogeneans
found on the gills were isolated and fixed with a
glycerin-ammonium picrate mixture and then remounted
in Canada balsam (Ergens, 1969). However,
in most cases this technique was modified using Berland's
solution before applying a glycerin—ammonium
picrate mixture (Berland, 1961). A worm was placed
in a small droplet of water on a slide, a small amount
of Berland's solution was added using a fine brush, and
the worm was covered with a coverslip. Excessive solution
was removed with filter paper. Each corner of
the coverslip was sealed with Du-Noyer sealant (Ergens
and Gelnar, 1992) and glycerin-ammonium picrate
solution was added to the edge of the coverslip.
Processed worms were remounted in Canada balsam
according to Ergens (1969).
In addition, some worms, fixed in 4% formalin,
were stained with Gomori's trichrome to study the
morphology of internal organs; others were mounted
unstained in glycerin jelly. All measurements are given
in micrometers; the mean is followed by the range and
number of specimens measured in parentheses. The
number of fishes infected of the total examined is followed
by the mean and the minimum and maximum
in parentheses. Drawings were made with the aid of
an Olympus drawing tube.
Specimens were deposited in the National Helminthological
Collection of Mexico, Institute of Biology,
National Autonomous University of Mexico (UNAM),
Mexico (CNHE); the United States National Parasite
Collection, Beltsville, Maryland, U.S.A. (USNPC); the
Institute of Parasitology, Academy of Sciences of the
Czech Republic, Ceske Budejovice, Czech Republic
(IPCAS); the Natural History Museum, London, United
Kingdom (BMNH); and the Laboratory of Parasitology,
Center for Research and Advanced Studies of
the National Polytechnic Institute (CINVESTAV-IPN),
Merida, Yucatan, Mexico (CHCM).
Results
Sciadicleithrum bravohollisae Kritsky,
Vidal-Martinez, and Rodriguez-Canul, 1994
MEASUREMENTS (based on 10 specimens from
Cichlasoma salvini (Giinther 1862)): Haptor
Copyright © 2011, The Helminthological Society of Washington
142 (120-190; n = 5) wide, 77 (62-105; n =
6) long. Pharynx spherical, 47 (39=65; n = 4)
in diameter. Ventral hamuli 29 (27-32; n = 18)
long; base width 17 (16-18; n = 18). Dorsal
hamuli 33 (31-34; n = 15) long; base width 15
(13-16; n = 15). Ventral bar 35 (29-45; n = 7)
long; dorsal bar 36 (31-44; n — 6) long. Hooks
15 (13-15; n — 20) long. Male copulatory organ
31 (25—36; n = 4) long. Accessory piece 20
(18-22; n = 6) long.
HOSTS, LOCALITIES, SAMPLING DATES, AND PA-
RAMETERS OF INFECTION: Cichlasoma geddesi
Regan, 1905, Horizonte Lagoon (17 November
1998), 1 fish infected of 1 examined; mean intensity
of infection 12 specimens; Cichlasoma
lentiginosum (Steindachner, 1864), Lacanja River
(21 May 1998), 2/3, 3 (minimum intensity 3,
maximum intensity 4); Cichlasoma managuense
(Gunther, 1869), Santa Gertrudis (17 March
1998), 1/1, 2; C. pearsei, Santa Gertrudis (17
March 1998), 1/7, 2; Lacanja River (21 May
1998), 2/3, (3-6); Palizada River (15 June
1998), 1/1, 4; C. salvini, Dzaptun Cenote (21
August 1996; 1 October 1997), 3/4, 2 (3-5), 51
5 (4-5); Lacanja River (21 May 1998), 1/1, 2;
Ilusiones Lagoon (16 November 1998), 5/10, 2
(1-3); Horizonte Lagoon (17 November 1998),
1/1, 19; Yumka Lake (18 November 1998), 1/3,
1; Puyacatengo River (19 November 1998), 21
10, 3 (1—6); C. synspilum, Cenote Azul (2 March
1998), 1/1, 6; Rancho II (17 March 1998), 1/6,
1; Raudales Lagoon (8 May 1998), 2/16, 14 (8-
21); Palizada River (15 June 1998), 1/6, 5; Cichlasoma
sp., Paraiso River (20 March 1998), 1/1, 5.
SPECIMENS DEPOSITED: Voucher specimens
from C. pearsei and C. synspilum in CHCM
(Nos. 215 and 214), from C. salvini in CNHE
(Nos. 3132 and 3133), IPCAS (No. M-348),
USNPC (Nos. 88946 and 88950), and CHCM
(Nos. 229 and 231).
REMARKS: Specimens found in C. salvini do
not differ from those of S. bravohollisae, as described
from C. pearsei, C. synspilum, and P.
splendida by Kritsky et al. (1994). The present
material enabled us to add some new data on the
morphology of the copulatory complex and the
vaginal aperture. Kritsky et al. (1994) reported
the length of the accessory piece to be 31—45
and 26-37 in specimens from C. pearsei and C.
synspilum, respectively. The present specimens
have the accessory piece considerably shorter,
measuring only 18—22.
The shape of the vaginal aperture is similar to