Comparative Parasitology 68(2) 2001 - Peru State College

Table 2. Prevalence (Pr) and
Rana clamitans melanota.
Trematoda
Species
Haematoloechus varioplexus
Halipegns eccentricus
Glypthelmins quieta
Unidentified metacercariae*
Diplostomid metacercariae*
Cestoda
Unidentified adult cestode
Mesocestoides sp.*
Nematoda
Oswaldocruzia pipiens
Cosmocercoides sp.
Waltonella sp.
Larval nematode
Encysted nematode
* Underestimate.
± Not counted.
BOLEK AND COGGINS—HELMINTH COMMUNITIES IN GREEN FROGS 167
mean intensity (MI) of helminth parasites in male and female green frogs,
Measure of
parasitism
Pr
MI ±
Pr
MI ±
PI-
MI ±
Pr
MI ±
Pr
MI ±
Pr
MI ±
Pr
MI ±
Pr
MI ±
Pr
MI ±
Pr
MI ±
Pr
MI ±
PI-
MI ±
1 SD
1 SD
1 SD
1 SD
1 SD
1 SD
1 SD
1 SD
1 SD
1 SD
1 SD
1 SD
first observed during midspring (May-June)
with a prevalence of 5%. Prevalence for this
species reached its maximum (30%) during summer
(July-August) and decreased during the fall
collection (20%). Seasonal mean intensity of
adult platyhelminths followed similar patterns as
prevalence, but no significant differences existed
for any of the adult platyhelminths recovered, H.
eccentricus (adjusted H = 2.02, P > 0.05), H.
varioplexus (F = 0.34, P > 0.05), or G. quieta
(t = 0.43, P > 0.05).
Although prevalence and intensity of H. varioplexus
did not vary significantly throughout
the collection period, mean length of worms did
(Fig. 1). Greatest mean length of worms (4.1
mm) was recorded in early spring (April), when
all individuals were gravid adults, and reached
a minimum during midspring (1.84 mm), when
immature individuals were common. Statistically
significant differences in mean length were
observed for April and May—June collections,
Males
N = 43
46.5 (20/43)
7 ± 8.6
23.3 (10/43)
2.3 ± 2.1
1 1 .6 (5/43)
21.8 ± 27.9
90.7 (39/43)
NC:!:
18.6 (8/43)
45.3 ± 46.1
2.3 (1/43)
1
23.3 (10/43)
24.9 ± 16.2
27.9 (12/43)
16.6 ± 25.8
20.9 (9/43)
2+1.7
0 (0/43)
0
9.3 (4/43)
11.5 ± 11.2
0 (0/43)
0
Females
N = 32
40.6 ( 1 3/32)
2.8 ± 2.2
21.9 (7/32)
1.4 ± 0.5
18.8 (6/32)
47 ± 38.1
65.6 (21/32)
NC
12.5 (4/32)
14.5 ± 23.7
0 (0/32)
0
12.5 (4/32)
49.3 ± 24.8
28.1 (9/32)
12.2 ± 19.6
31.3 (10/32)
4.4 ± 5.6
3.1 (1/32)
4
18.8 (6/32)
35.5 ± 80.6
3.1 (1/32)
1
Statistic
X2 = 0.24
f',= 3.07
X2 = 0.02
f',= 2.71
X2 = 0.46
t = 1 .35
X2 = 5.72
X2odj = 0.16
t = 1.23
X2ailJ = 0.02
X2adj = 0.78
t = 6.69
x2 = o.oo
/ = 0.44
X2 = LOO
r',= 3.81
X2ailj = 0.02
X2:,Ji = 1-67
f',= 1.67
X2a(lj = 0.02
P
>0.05
0.05
0.05
>0.05
0.05
>0.05
>().05
>0.05
0.05
>0.05
>0.05
0.05
>0.05
>0.05
>0.05
April and July-August collections, May-June
and September—October collections, and July-
August and September-October collections (F =
11.8, P < 0.05, single-factor, independent-measure
ANOVA; P < 0.05 for all pair-wise comparisons,
Scheffe's test).
The nematodes Waltonella sp. and an unidentified
encysted larva were recovered infrequently
as single infections during midspring and fall
collections, respectively. Prevalence and intensity
values for O. pipiens, Cosmocercoides sp.,
and unidentified larval nematodes were low and/
or erratic over the 7-mo period. The nematodes
O. pipiens, Cosmocercoides sp., and unidentified
larval nematodes were first observed during
midspring and persisted until fall, with prevalence
being highest in summer for O. pipiens
(62%) and midspring and summer for Cosmocercoides
sp. (40%) and larval nematodes
(20%). However, only O. pipiens exhibited statistically
significant differences (adjusted H =
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