Comparative Parasitology 68(2) 2001 - Peru State College

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Comp. Parasitol. 68(2), 2001, pp. 236-241 Supplemental Diagnosis of Myxobolus gibbosus (Myxozoa), with a Taxonomic Review of Myxobolids from Lepomis gibbosus (Centrarchidae) in North America DAVID K. CONE Department of Biology, Saint Mary's University, Halifax, Nova Scotia, Canada B3H 3C3 (e-mail: david.cone@stmarys.ca) ABSTRACT: Myxobolus gibbosus Herrick, 1941 (Myxosporea) is reported from the connective tissue of gills of Lepomis gibbosus (Centrarchidae) in Algonquin Park, Ontario, Canada. The new material (formalin-preserved) is used to supplement the original taxonomic diagnosis of nearly 60 yr ago. Spores are round to oval in valvular view, 11-14 jjim long and 10-11 jxm wide, with a distinctly blunt capsular region. The polar capsules are relatively large for the size of the spore, measuring 6-7 jxm long and 3.5-4.0 (Jim wide, and aligned almost parallel to each other. There are 8-12 loose filament coils lying up to 45° to the long axis of the capsule. The taxonomy of species of Myxobolus described or reported from L. gibbosus in North America is examined, and the following are considered to be valid taxa: Myxobolus dechtiari Cone and Anderson, 1977; M. gibbosus; Myxobolus magnasphcrus Cone and Anderson, 1977; Myxobolus osburni Herrick, 1936; Myxobolus paralintoni Li and Desser, 1985; and Myxobolus uvidiferus Cone and Anderson, 1977. Comparative photographs of spores accompany differential diagnoses of the 6 species. Myxobolus gibbosus Li and Desser, 1985, and Myxobolus Hi Desser, 1993, are junior synonyms of M. uvidiferus. Myxobolus lepomicus Li and Desser, 1985, is considered a species inquirendae, and the reports of Myxobolus cyprinicola Reuss, 1906, and Myxobolus poecilichthidis Fantham, Porter, and Richardson, 1939, from L. gibbosus are considered misidentifications. KEY WORDS: Myxobolus gibbosus, Myxosporea, redescription, differential diagnoses, pumpkinseed sunfish, Lepomis gibbosus, Centrarchidae, Algonquin Park, Canada. Myxobolus gibbosus Herrick, 1941 (Myxozoa) was described from connective tissue of the gill arch of pumpkinseed sunfish (Lepomis gibbosus (Linnaeus, 1758)) from the island region of western Lake Erie (Herrick, 1941). In subsequent surveys of myxosporean parasites of pumpkinseed (Cone and Anderson, 1977a, b; Li and Desser, 1985; Hayden and Rogers, 1997), the parasite was not encountered. However, during a new survey of myxosporeans of fish in Algonquin Park, a single pseudocyst of M. gibbosus was discovered. This rare find enabled the author to assess information provided in the original species description and to critically compare the parasite with other species of the genus reported from pumpkinseed. The present study describes the new material and reviews the taxonomy of myxobolids from pumpkinseed in North America. Materials and Methods Nine pumpkinseed (6-8.9 cm in total length) were collected in baited trapnets set 20 June 1994 and 21 June 1995 in the shallows of Lake Sasajewan (45°35'N; 78°30'W), Algonquin Park, Ontario, Canada. The fish were pithed and necropsied. All body organs and tissues were examined microscopically for myxosporean pseudocysts, and, when found, they were 236 fixed in 10% buffered formalin. Fixed pseudocysts were punctured and the spore contents stabilized in temporary mounts prepared with 1% agar (Lom, 1969). Spores were photographed with interference contrast optics. Enlarged photographic prints of individual spores were used to determine spore dimensions. Descriptive terminology follows Lom and Dykova (1992). Measurements are presented in micrometers. The sample of M. gibbosus was compared with other species of Myxobolus in the author's collection, namely Myxobolus dechtiari Cone and Anderson, 1977; Myxobolus magnaspherus Cone and Anderson, 1977; Myxobolus osburni Herrick, 1936; Myxobolus paralintoni Li and Desser, 1985; and Myxobolus uvitliferus Cone and Anderson, 1977. Syntype slides of M. gibbosus (NMCICP 1984-0359), Myxobolus lepomicus (NMCICP 1984-0362), and M. paralintoni (NMCICP 1984-0364) housed in the parasite collection of the Canadian Museum of Nature were also examined. A photo-voucher (negative film) is deposited in the United States National Parasite Collection (USNPC), Beltsville, Maryland, U.S.A. Results Myxobolus gibbosus Herrick, 1941 (Figs. 1 and 2) Supplementary diagnosis Copyright © 2011, The Helminthological Society of Washington Pseudocyst egg-shaped, gray-white and minute (250 long), embedded in connective tissue surrounding base of gill arch. Spores round to
Figure 1. Spores of Myxobolus gibbosus. Scale bar = 10 [Jim. oval in valvular view, with blunt capsular edge. Spores 11.8 ± 0.9 (11-14, « = 10) long and 10.6 ± 0.5 (10-1 1) wide. Width-to-length ratio 1:1.09 ± 0.08 (1.05-1.3). Polar capsules oval, 6.8 ± 0.3 (6-7) long and 4.0 ± 0.3 (3.5-4) wide, aligned almost parallel to each other. Polar filaments in 8-12 loose coils, lying up to 45° to long axis of capsule. Capsulogenic nuclei prominent, triangular. Shallow intercapsular appendix evident in some spores. Sutural ridge thin and smooth. Taxonomic summary HOST: Pumpkinseed sunfish (Lepomis gibbosus) (Centrarchidae); total length 6.2 cm, 1 + yr old. LOCALITY/COLLECTION DATE: Lake Sasajewan, Algonquin Park, Ontario, Canada (45°35'N; 78°30'W), 20 June 1994. CONE—DIAGNOSIS OF MYXOBOLUS 237 SITE OF INFECTION: Connective tissue of gill arch. PREVALENCE AND INTENSITY OF INFECTION: One of 9 fish infected with 1 pseudocyst. SPECIMENS DEPOSITED: Photo-voucher USNPC No. 091157.00. Remarks Myxobolus gibbosus has not been reported in surveys of myxozoans in L. gibbosus from Algonquin Park (Cone and Anderson 1977a, b; Li and Desser, 1985). It was probably not overlooked, for the parasite has several distinct diagnostic features. It forms small but obvious pseudocysts in the connective tissue of the gill arch and produces round spores with a blunt capsular end. The polar capsules are relatively large, the length being about half the length of the spore, and they are arranged almost parallel Copyright © 2011, The Helminthological Society of Washington
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Volume 68 July 2001 Number 2 Former
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Comp. Parasitol. 68(2), 2001, pp. 1
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CRISCIONE AND FONT—DEVELOPMENT OF
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length did not exceed 1.2 mm. By da
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Agamidae) from Australia. Journal o
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infection with O. javaensis (C. D.
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CRISCIONE AND FONT—VARIATION OF O
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CRISCIONE AND FONT—VARIATION OF O
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and Font, 2001), the indication of
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U.S.A. (42°59'N; 88°21'W). Ten to
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Table 2. Prevalence (Pr) and Rana c
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transmission dynamics of these 3 tr
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196 in G. W. Esch, A. O. Bush, and
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filariae of S. fallisensis was simi
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CARRENO ET AL.—DEER PARASITES IN
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ange of host species, but most coll
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Oestridae). Canadian Journal of Zoo
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Page 43 and 44: south-central New York salamanders.
Page 45 and 46: AGUIRRE-MACEDO ET AL.—ENDOHELMINT
Page 51 and 52: Table 1. Helminths found in freshwa
Page 53 and 54: •o d c o U rH CB 3 C8 H °Q 0 W T
Page 55 and 56: da-Lopez et al., 1985; Leon, 1992;
Page 57 and 58: especie del genero Goezici Zeder, 1
Page 59 and 60: JALISCO SALGADO-MALDONADO ET AL.—
Page 61 and 62: SALGADO-MALDONADO ET AL.—HELMINTH
Page 63 and 64: Table 3. Continued. Number of hosts
Page 71 and 72: tudio helmintologico de Chirostoma
Page 75 and 76: CURRAN ET AL.—TWO VIETNAMESE SNAK
Page 77 and 78: methods of fixation had not been kn
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Page 81 and 82: tylidae Poche: Part 2. A contributi
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Page 89: Literature Cited Baker, M. R. 1978.
Page 93 and 94: CONE—DIAGNOSIS OF MYXOBOLUS 239 F
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Page 127 and 128: (Nematoda). Except for H. tursionis
Page 129 and 130: RESEARCH NOTES 275 Figure 1. Cephal
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Page 135 and 136: ticeps (Rudolphi, 1819), which also
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Adenomera hylaedactyla, 21 Algansea
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Honorary Membership, 282 Life Membe
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Schrankiana larvata, 21 Schrankiana
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*Edna M. Buhrer "Mildred A. Doss *
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