Several studies indicated that obese subjects, particularly those with visceral fat accumulation, have reduced plasma levels of adiponectin. Other results indicate that rs1049353 (1359G/A) polymorphism of the CNR1 gene is associated with increased abdominal circumference (AC). The aim of our study was to investigate the association between the rs1049353 polymorphism and low adiponectin levels in subjects with abdominal obesity (AO). The study included 377 subjects divided in: 219 subjects with AO (AC≥102cm in men; AC≥88cm in women) and 158 subjects without AO. The groups were divided in 2 subgroups: one with low adiponectin levels (
Integrated Physiology/ Obesity POSTERS within an intron of DDX60L which encodes a ATP-dependent helicase binding with RNA. CD83 and RNF182 are the only 2 genes within ±500kb of rs6459353. CD83 is a cell-surface glycoprotein that regulates immunity response, and RNF182 is a brain-enriched E3 ubiquitin ligase that may participate in controlling the neurotransmitter release mechanism. To explore potential associations between these 3 genes and BMI, the putative promoter regions and all exons and exon-intron boundaries of these genes were sequenced in 24 Pima Indians. 4 novel SNPs were identifi ed (2 in DDX60L, 1 in CD83 and 1 in RNF182); one novel SNP within DDX60L predicts a 1323F/I substitution and the other 3 SNPs are in untranslated regions. These SNPs, in addition to 53 tag SNPs from the HapMap (CHB) are currently being genotyped in our samples. 1926-P Vitamin D Insuffi ciency, a Risk Factor for Prediabetes and <strong>Diabetes</strong> Type 2 in Abdominal Obesity Women TATIANA KARONOVA, ELENA MICHEEVA, DANY AZZI, ELENA KONOPLYANNI- KOVA, IDELYA MAMINA, ELENA KRASILNIKOVA, EUGENIYA PATRAKEEVA, ELENA GRINEVA, EUGENIY SHLYAKHTO, St. Petersburg, Russia Some studies suggest that serum 25(OH) vitamin D concentration is inversely associated with insulin resistance and the prevalence of diabetes type 2. However due to the uncertainty of these associations, we examined the relationship between serum concentration of 25(OH)D and glucose as well as insulin levels, HOMA-IR, and ISI (0,120) in abdominal obesity women. We examined 162 women 40 to 52 years old (mean age 46.9±5.5) with abdominal obesity (according to IDF criteria, 2005). The mean waist circumference was 96.5±1.7 cm, body mass index (BMI) was 31.3±0.4 kg/m 2 , waist-to-hip ratio (WHR) - 0.9±0.004. Serum 25(OH)D and insulin levels were determined by ELISA, plasma glucose levels - by standard biochemistry. All patients underwent standard oral glucose-tolerance test (75 g of glucose). Insulin resistance was evaluated by HOMA-IR and insulin sensitivity by ISI (0, 120). Glucose level was 6.1±0.2 mMol/L, insulin level 14.9±0.9 (mIU/ml), HOMA- IR - 3.2±0.1, ISI (0, 120) – 7.52±0.39. Serum 25(OH)D level was from 19.4 to 134.1 nMol/L (mean 55.6±2.9). Vitamin D insuffi ciency and defi ciency was revealed in 83.6% of patients, only 16.4% women had normal 25(OH)D level. Glucose intolerance was revealed in 44 women (25.9%), diabetes type 2 was diagnosed in 25 women (16.1%). Correlation analysis showed inversed correlations between serum 25 (OH)D level and fasting glucose, insulin levels (r=0.14, p=0.05; r=0.20, p=0.008 respectively) and ISI (0,120) (r=0.33, p=0.001). We found that HOMA-IR was related to BMI value (r=0.5, p=0.001), but not with 25(OH)D levels (r=-0.14, p=0.08). Our results showed that women with abdominal obesity have higher tendency for developing vitamin D defi ciency and this later is correlated with high fasting plasma glucose, serum insulin levels and decreased tissues insulin sensitivity. Hence vitamin D insuffi ciency might possibly be a risk factor for pre diabetes and diabetes type 2. 1927-P Vitamin D-Associated Polymorphisms Are Related to Insulin Resistance and Vitamin D Defi ciency in Polycystic Ovary Syndrome WITHDRAWN ELISABETH WEHR, THOMAS R. PIEBER, BARBARA OBERMAYER-PIETSCH, Graz, Austria Women with polycystic ovary syndrome (PCOS) frequently suffer from insulin resistance, which might be related to vitamin D metabolism. We aimed to investigate the association of polymorphisms in the vitamin D receptor (VDR) gene and vitamin D level associated genes with metabolic and endocrine parameters in PCOS and control women. Metabolic, endocrine, and anthropometric measurements and oral glucose tolerance tests were done in 545 PCOS and 145 control women. Genotyping of VDR (Cdx-2, Bsm-I, Fok-I, Apa-I, and Taq-I), GC, DHCR7, and CYP2R1 polymorphisms was performed. 25-hydroxyvitamin D (25[OH]D) levels correlated negative with insulin resistance and positive with insulin sensitivity (p
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