2011 ADA Posters 1261-2041.indd - Diabetes

Integrated Physiology/
Obesity
POSTERS
within an intron of DDX60L which encodes a ATP-dependent helicase
binding with RNA. CD83 and RNF182 are the only 2 genes within ±500kb
of rs6459353. CD83 is a cell-surface glycoprotein that regulates immunity
response, and RNF182 is a brain-enriched E3 ubiquitin ligase that may
participate in controlling the neurotransmitter release mechanism. To
explore potential associations between these 3 genes and BMI, the putative
promoter regions and all exons and exon-intron boundaries of these genes
were sequenced in 24 Pima Indians. 4 novel SNPs were identifi ed (2 in
DDX60L, 1 in CD83 and 1 in RNF182); one novel SNP within DDX60L predicts a
1323F/I substitution and the other 3 SNPs are in untranslated regions. These
SNPs, in addition to 53 tag SNPs from the HapMap (CHB) are currently being
genotyped in our samples.
1926-P
Vitamin D Insuffi ciency, a Risk Factor for Prediabetes and Diabetes
Type 2 in Abdominal Obesity Women
TATIANA KARONOVA, ELENA MICHEEVA, DANY AZZI, ELENA KONOPLYANNI-
KOVA, IDELYA MAMINA, ELENA KRASILNIKOVA, EUGENIYA PATRAKEEVA,
ELENA GRINEVA, EUGENIY SHLYAKHTO, St. Petersburg, Russia
Some studies suggest that serum 25(OH) vitamin D concentration is
inversely associated with insulin resistance and the prevalence of diabetes
type 2. However due to the uncertainty of these associations, we examined
the relationship between serum concentration of 25(OH)D and glucose as well
as insulin levels, HOMA-IR, and ISI (0,120) in abdominal obesity women.
We examined 162 women 40 to 52 years old (mean age 46.9±5.5) with
abdominal obesity (according to IDF criteria, 2005). The mean waist circumference
was 96.5±1.7 cm, body mass index (BMI) was 31.3±0.4 kg/m 2 , waist-to-hip ratio
(WHR) - 0.9±0.004. Serum 25(OH)D and insulin levels were determined by
ELISA, plasma glucose levels - by standard biochemistry. All patients underwent
standard oral glucose-tolerance test (75 g of glucose). Insulin resistance was
evaluated by HOMA-IR and insulin sensitivity by ISI (0, 120).
Glucose level was 6.1±0.2 mMol/L, insulin level 14.9±0.9 (mIU/ml), HOMA-
IR - 3.2±0.1, ISI (0, 120) – 7.52±0.39. Serum 25(OH)D level was from 19.4 to
134.1 nMol/L (mean 55.6±2.9). Vitamin D insuffi ciency and defi ciency was
revealed in 83.6% of patients, only 16.4% women had normal 25(OH)D level.
Glucose intolerance was revealed in 44 women (25.9%), diabetes type 2
was diagnosed in 25 women (16.1%). Correlation analysis showed inversed
correlations between serum 25 (OH)D level and fasting glucose, insulin
levels (r=0.14, p=0.05; r=0.20, p=0.008 respectively) and ISI (0,120) (r=0.33,
p=0.001). We found that HOMA-IR was related to BMI value (r=0.5, p=0.001),
but not with 25(OH)D levels (r=-0.14, p=0.08).
Our results showed that women with abdominal obesity have higher
tendency for developing vitamin D defi ciency and this later is correlated with
high fasting plasma glucose, serum insulin levels and decreased tissues
insulin sensitivity. Hence vitamin D insuffi ciency might possibly be a risk
factor for pre diabetes and diabetes type 2.
1927-P
Vitamin D-Associated Polymorphisms Are Related to Insulin Resistance
and Vitamin D Defi ciency in Polycystic Ovary Syndrome
WITHDRAWN
ELISABETH WEHR, THOMAS R. PIEBER, BARBARA OBERMAYER-PIETSCH, Graz,
Austria
Women with polycystic ovary syndrome (PCOS) frequently suffer from
insulin resistance, which might be related to vitamin D metabolism. We
aimed to investigate the association of polymorphisms in the vitamin D
receptor (VDR) gene and vitamin D level associated genes with metabolic
and endocrine parameters in PCOS and control women.
Metabolic, endocrine, and anthropometric measurements and oral
glucose tolerance tests were done in 545 PCOS and 145 control women.
Genotyping of VDR (Cdx-2, Bsm-I, Fok-I, Apa-I, and Taq-I), GC, DHCR7, and
CYP2R1 polymorphisms was performed.
25-hydroxyvitamin D (25[OH]D) levels correlated negative with insulin
resistance and positive with insulin sensitivity (p