Dipropylene glycol (SIDS)
Dipropylene glycol (SIDS)
Dipropylene glycol (SIDS)
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OECD <strong>SIDS</strong> PROPYLENE GLYCOL<br />
5. Toxicity<br />
Id 25265-71-8<br />
Date 30.05.2001<br />
Concentration : 29-60 ul/ml without activation; 30 - 50 ul/ ml with activation<br />
Cycotoxic conc. : not reported<br />
Metabolic activation : with and without<br />
Result : negative<br />
Method : other: Suspension/plate<br />
Year : 1987<br />
GLP : no data<br />
Test substance : no data<br />
Method : Limited details reported.<br />
Result : No increase in revertants was recorded for dipropylene <strong>glycol</strong> with or<br />
without activation.<br />
Source : Lyondell Chemical Co. Houston, Texas<br />
Reliability : (2) valid with restrictions<br />
summary data only available from NCI program.<br />
Flag : Critical study for <strong>SIDS</strong> endpoint<br />
29.05.2001 (11)<br />
5.6 GENETIC TOXICITY ‘IN VIVO‘<br />
Type : Micronucleus assay<br />
Species : mouse<br />
Sex : male<br />
Strain : other: CD-1(ICR)BR<br />
Route of admin. : gavage<br />
Exposure period : 2 days<br />
Doses : 500, 1000 and 2000 mg/kg<br />
Result : negative<br />
Method : other: US EPA OPPTS 870.5395 and OECD 474<br />
Year : 1999<br />
GLP : yes<br />
Test substance : as prescribed by 1.1 - 1.4<br />
Method : Animals and treatments<br />
Eight week old male CD-1 mice from Charles River Labs were used in<br />
these investigations. Six mice were used per treatment group (control,<br />
positive control, 3 levels of dipropylene <strong>glycol</strong>). The dose levels were based<br />
on a preliminary study. <strong>Dipropylene</strong> <strong>glycol</strong> was administered for two<br />
consecutive days by gavage at 500, 1000 or 2000 mg/kg/day in water at a<br />
dose volume of 10 ml/kg. 120 mg/kg/day cyclophophamide was<br />
administered on two consecutive days by gavage at a dose volume of 10<br />
ml/kg.<br />
Preparation and examination of bone marrow smears<br />
Mice were killed by carbon dioxide inhalation 24 hr after second gavage<br />
dose of dipropylene <strong>glycol</strong>. Femoral marrow cells were isolated, smeared<br />
onto clean glass slides, fixed with methanol and stained with Wright-<br />
Giemsa. The preparations were coded and analyzed without identification<br />
of animal number or treatment. Two thousand polychromatic erythrocytes<br />
(PCEs) per mouse were examined using light microscopy (x100), and the<br />
number of micronucleated polychromatic erythrocytes (MNPCEs) was<br />
recorded. The proportion of PCEs among the total erythrocytes was also<br />
evaluated by observation of 200 erythrocytes on the same slide.<br />
Positive control substance<br />
120 mg/kg/day cyclophosphamide administered two consecutive days by<br />
gavage.<br />
Statistical analysis<br />
The frequency of MNPCEs in each treatment was analyzed by one-way<br />
72<br />
UNEP PUBLICATIONS
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