Dipropylene glycol (SIDS)

OECD SIDS PROPYLENE GLYCOL
5. Toxicity
Id 25265-71-8
Date 30.05.2001
Method : Species: rat
Sex: male
Strain: F344
Number of a nimals: 10
Route of admin.: gavage
Exposure period: one dose
Frequency of treatment: once
Duration of test: 24 hrs
Doses: 40 mg/kg/day TPG in water at dosing volume of 4 ml/kg.
Control group: No
Year: 1995
GLP: yes
Animals and treatment
Male F344 rats weighing between 187 and 197 g were obtained from
Charles River and allowed 2 days for acclimation in metabolism cages
before dosing. After dosing, the animals were held in the metabolism
cages for 24 hours.
Sample collections
Air drawn through the metabolism chambers was passed through a trap for
collection of radioactive CO2. All voided urine was collected and frozen for
analysis. Feces were collected in dry-ice chilled containers for analysis.
After 24 hours the animals were sacrificed for collection of blood, liver,
kidney, fat, brain, muscle, and the remaining carcass.
Sample analysis
Radioactivity in all collected samples was determined using a liquid
scintillation counter. Urine samples were analyzed by gas chromatography
for tripropylene glycol, dipropylene glycol and propylene glycol following
derivatization to pentafluorobenzoyl esters.
Source : Lyondell Chemical Co. Houston, Texas
Test substance : Analytical data verified identity: 99.8% tripropylene glycol., 0.08%
dipropylene glycol pure. 14C-labelled test material TM = 14C-labelled
TPG.
Conclusion : Result: 91.4±2.1% of the administered dose was recovered from CO2,
urine, feces, tissues and carcass. 20.7±0.6% was recovered as CO2;
52.3±3.5% was recovered in urine. Approximately 10% of the administered
radioactivity remained in the carcass after 24 hours.
Urine analysis
13% of the administered dose of tripropylene glycol was recovered in the
urine as free or conjugated tripropylene glycol, 8.4% as free or conjugated
dipropylene glycol, and 3.9% as free or conjugated propylene glycol.
Administration of propylene glycol resulted in 12% recovered as propylene
glycol in the urine.
Conclusion: Tripropylene glycol is rapidly and extensively metabolized to
dipropylene glycol which is further metabolized to propylene glycol and
subsequently to lactic or pyruvic acids followed by oxidation through the
citric acid cycle (21% to CO2) or production of glycogen. Much of the
radioactivity remaining in the carcass probably represented metabolically
incorporated carbons atoms derived from propylene glycol. (author
conclusion)
Reliability : (1) valid without restriction
meets guidelines and GLP.
Flag : Critical study for SIDS endpoint
29.05.2001 (18)
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