Flavonoid-Drug Interactions: Effects of Flavonoids on ABC ...

Effect <strong>of</strong> <strong>Flavonoid</strong>s on Topotecan
Accumulation in MCF-7 cells
Accumulation time: 10 min;
Topotecan concentration: 5 µM;
Cells were harvested and
sonicated
Topotecan in cell lysate was
assayed by HPLC
Accumulation were normalized
by protein content
N = 4
Topotecan accumulation
(% <strong>of</strong> control)
350
300
250
200
150
100
50
0
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control
10 µM PSC833
10 µM FTC
10 µM PSC833 + 10 µM FTC
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50 µM chrysin
5 µM BF
Chrysin and BF can inhibit BCRP-mediated
efflux <strong>of</strong> topotecan
MCF-7 MX100
MCF-7/sensitive