E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...
E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...
E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...
- No tags were found...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
RING domain of <strong>TRIM72</strong> is required for <strong>IRS</strong>-1 degradation.<br />
- - - -<br />
+ + + +<br />
-<br />
MG132<br />
Flag-h<strong>IRS</strong>-1<br />
HA-h<strong>TRIM72</strong><br />
IB : Flag<br />
IB : HA<br />
140<br />
- MG132<br />
- - + +<br />
- + - +<br />
+ MG132<br />
- - + +<br />
- + - +<br />
Flag-h<strong>IRS</strong>-1<br />
HA-h<strong>TRIM72</strong><br />
IB: Flag<br />
IB: HA<br />
<strong>IRS</strong>-1 Expression<br />
Level (%)<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
+ + + +<br />
-<br />
+<br />
-<br />
+<br />
- - + +<br />
Flag-h<strong>IRS</strong>-1<br />
HA-h<strong>TRIM72</strong><br />
MG132<br />
HEK 293 cells
![Microsoft PowerPoint - src.ppt [\310\243\310\257 \270\360\265\345]](https://img.yumpu.com/47977670/1/190x134/microsoft-powerpoint-srcppt-310243310257-270360265345.jpg?quality=85)
![Microsoft PowerPoint - src.ppt [\310\243\310\257 \270\360\265\345]](https://img.yumpu.com/44373305/1/190x134/microsoft-powerpoint-srcppt-310243310257-270360265345.jpg?quality=85)
Change language