E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...
E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...
E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...
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RING domain of <strong>TRIM72</strong> is required for <strong>IRS</strong>-1 ubiquitination.<br />
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HA-TRIM<br />
HA-C14A<br />
HA-∆R<br />
Flag-<strong>IRS</strong>-1<br />
His-Ub<br />
IP, α-Flag; IB, α-His<br />
IP, α-Flag; IB, α-Flag<br />
IB: α-HA (WCL)<br />
IB: α-Flag (WCL)<br />
In the presence of MG132<br />
HEK 293 cells