23.01.2015 Views

E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...

E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...

E3 ligase TRIM72 negatively regulates myogenesis by IRS-1 ...

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RING domain of <strong>TRIM72</strong> is required for <strong>IRS</strong>-1 ubiquitination.<br />

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+<br />

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HA-TRIM<br />

HA-C14A<br />

HA-∆R<br />

Flag-<strong>IRS</strong>-1<br />

His-Ub<br />

IP, α-Flag; IB, α-His<br />

IP, α-Flag; IB, α-Flag<br />

IB: α-HA (WCL)<br />

IB: α-Flag (WCL)<br />

In the presence of MG132<br />

HEK 293 cells

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