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Investigating carotenoid loss after drying and storage of

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194<br />

8. Study under controlled conditions<br />

• There was also a linear relationship between !-carotene degradation rate <strong>and</strong> water<br />

activity, <strong>and</strong> between !-carotene degradation rate <strong>and</strong> oxygen concentration. This<br />

relationship requires further research but highlighted the straightforward influence <strong>of</strong><br />

these factors on <strong>carotenoid</strong> degradation. Oxygen (0-21%) with degradation constant<br />

rates ranging between 0.0143-0.0887 day -1 has a far greater influence on <strong>carotenoid</strong><br />

degradation than water activity (0.13-0.76) with variations between 0.0332-0.0496<br />

day -1 . Temperature (10-40ºC) has also an important influence on !-carotene<br />

degradation with a range <strong>of</strong> 0.0024-0.0403 day -1 .<br />

• Norisoprenoid formation (!-ionone; 5,6 epoxy-!-ionone; DHA) during <strong>storage</strong> <strong>of</strong><br />

dried OFSP chips was clearly related to the corresponding degradation <strong>of</strong> !-carotene.<br />

The higher, the !-carotene degradation, the higher was the norisoprenoid formation.<br />

At higher water activities, !-carotene was better preserved <strong>and</strong> lower concentrations<br />

<strong>of</strong> volatiles were recorded. A similar observation was made at lower oxygen levels.<br />

• With the identification <strong>of</strong> norisoprenoids products <strong>and</strong> their variation <strong>of</strong><br />

concentration during <strong>storage</strong>, a potential pathway <strong>of</strong> degradation was proposed. This<br />

pathway could be investigated using statistical prediction tools to predict the<br />

concentration <strong>of</strong> norisoprenoid compounds during <strong>storage</strong>. Further research is<br />

required to underst<strong>and</strong> nature <strong>of</strong> the intermediate compounds between !-carotene <strong>and</strong><br />

norisoprenoids formed during <strong>storage</strong> <strong>and</strong> the kinetics <strong>of</strong> their formation <strong>and</strong><br />

degradation.

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