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Investigating carotenoid loss after drying and storage of

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85<br />

3. Preliminary study<br />

Air velocity was 0.04m.s -1 . Solar irradiance (Pyranometer Cimel CE 180 (Paris,<br />

France)) ranged between 421 <strong>and</strong> 1005 W.m -2 (9 am to 2 pm) depending on the course<br />

<strong>of</strong> the sun with an average <strong>of</strong> 751 W.m -2 . Temperature <strong>and</strong> humidity as well as air<br />

velocity through sample are presented in Figure 3-4. Tray loading densities were 3.5<br />

kg.m -2 for both chips <strong>and</strong> crimped slices.<br />

Open air sun-<strong>drying</strong> was carried out concurrently with solar-<strong>drying</strong> <strong>and</strong> using the same<br />

tray loading density (Table 1). Wire mesh trays (0.43x 0.45 m) were placed in the sun on<br />

a st<strong>and</strong> 10 cm above ground level.<br />

3.2.6 Dimensions <strong>of</strong> chip <strong>and</strong> slice samples<br />

Three photographs <strong>of</strong> samples (chips <strong>and</strong> slices together) in open air <strong>drying</strong> at the start<br />

<strong>of</strong> <strong>drying</strong>; <strong>after</strong> two hours <strong>and</strong> at the end <strong>of</strong> <strong>drying</strong> were analysed using Image J 1.40g<br />

S<strong>of</strong>tware (National Institute <strong>of</strong> Health, USA) (Figure 3-5).<br />

Figure 3-5: Crimp <strong>and</strong> grated chips before/<strong>after</strong> open-sun-<strong>drying</strong><br />

Using the width <strong>of</strong> the <strong>drying</strong> tray as known measurement, pixels values were converted<br />

into distance units (cm) (11 pixels=1 cm in the three photographs). On each picture<br />

thirty chips <strong>and</strong> slices were selected individually <strong>and</strong> their visible surface area calculated<br />

using ROI (Region <strong>of</strong> Interest) manager macro in Image J s<strong>of</strong>tware. Area measured<br />

using the Image J s<strong>of</strong>tware was in agreement with earlier estimation by calliper<br />

measurement (0.01 mm precision) done on ten chips/slices at initial time.<br />

3.2.7 Moisture <strong>and</strong> water activity determination<br />

Dry matter contents were determined by <strong>drying</strong> triplicate 5 g samples at 105ºC to<br />

constant mass (AOAC 1984). Water activity (aw) was determined in duplicate on finely

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