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Investigating carotenoid loss after drying and storage of

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205<br />

9. Discussion<br />

Absence <strong>of</strong> cis-isomerisation was further observed during the <strong>storage</strong> <strong>of</strong> dried sweet<br />

potato chips. During the <strong>storage</strong> <strong>of</strong> dried samples at ambient temperature in the dark (ca.<br />

25ºC) in Mozambique for four months (Chapter 5), trans-!-carotene, 9-cis <strong>and</strong> 13-cis all<br />

degraded following first order kinetics.<br />

As opposed to light, temperature during <strong>storage</strong> was shown to have a very significant<br />

impact on <strong>carotenoid</strong> catabolism <strong>of</strong> dried sweet potato in accordance with literature<br />

(Cinar 2004; Kósambo 2004; Koca et al. 2007; Hidalgo <strong>and</strong> Br<strong>and</strong>olini 2008) (Chapter<br />

8). It was also shown that <strong>carotenoid</strong> content <strong>of</strong> samples <strong>of</strong> dried OFSP stored in the<br />

freezer (-20ºC) for four months remained constant (Chapter 2).<br />

The effect <strong>of</strong> temperature (10-40ºC) that can be found in tropical <strong>and</strong> sub-Saharan<br />

climates such as those <strong>of</strong> developing countries was evaluated during <strong>storage</strong>.<br />

Carotenoids degraded following a first order kinetics <strong>of</strong> degradation in accordance with<br />

many authors including Koca et al. (2007); Lavelli et al. (2007) working on dehydrated<br />

carrots; Hidalgo <strong>and</strong> Br<strong>and</strong>olini (2008) working on wheat flour. A predictive model<br />

(Arrhenius model) developed in laboratory was further assessed with data from the field<br />

in Ug<strong>and</strong>a (Chapter 8). This model was able to successfully predict the <strong>carotenoid</strong><br />

degradation as affected by temperature <strong>and</strong> <strong>storage</strong> time <strong>and</strong> could be used as a tool to<br />

evaluate sweet potato dried chips shelf life.<br />

9.5.3 Water activity<br />

The lowest degradation <strong>of</strong> <strong>carotenoid</strong>s occurred at the highest water activity level in<br />

accordance with the studies on food model <strong>and</strong> on dehydrated sweet potato (Haralampu<br />

<strong>and</strong> Karel 1983) <strong>and</strong> was explained by the protective effect <strong>of</strong> water against air oxidation<br />

(Chou <strong>and</strong> Breene 1972). Water activity is an important parameter for the measurement<br />

<strong>of</strong> food quality <strong>and</strong> preservation. Moreover water activity was proved to influence<br />

<strong>carotenoid</strong> content in the <strong>storage</strong> <strong>of</strong> foodstuff (carrots (Arya et al. 1979; Lavelli et al.<br />

2007); sweet potato (Haralampu <strong>and</strong> Karel 1983)) <strong>and</strong> β-carotene food model systems<br />

(Chou <strong>and</strong> Breene 1972; Goldman et al. 1983) (Chapter 8). As compared with water<br />

activity, temperature <strong>and</strong> oxygen had a stronger effect on <strong>carotenoid</strong> degradation<br />

(Chapter 8).

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