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MANUAL PHYSIOLOGY PRACTICAL - Repository:The Medical ...

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FM/UOJ<br />

Explain why the red and white cells are separated:<br />

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PCV of capillary blood: - Method<br />

Perform finger pick and collect blood into a heparinized glass capillary tube to about<br />

75% of its length without any air bubble. Cover the end, through which blood was collected,<br />

by a finger and hold the open end on to a source of heat (spirit lamp) in order to seal the tip.<br />

Once the tip is sealed keep the tube in a groove, the sealed end facing outwards, in the micro<br />

centrifuge and note the number. <strong>The</strong> centrifuge will be switched on when sufficient number<br />

of capillary tubes are loaded and centrifuged for five minutes. After centrifugation, take your<br />

tube and read the percentage of packed cells with the help of the Micro-haematocrit reader<br />

(instruction for using the reader is found on the back of the reader).<br />

Precautions:<br />

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Blood<br />

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