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MANUAL PHYSIOLOGY PRACTICAL - Repository:The Medical ...

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FM/UOJ<br />

Calculation:<br />

<strong>The</strong> volume of the fluid in medium square<br />

Volume of fluid in all 5 squares<br />

= 0.004 sq.mm.<br />

= 5 x 0.004 cu.mm<br />

= 0.02 cu.mm.<br />

Let the total number of cells counted in all 5 areas be C<br />

Number of cells in 1 cu.mm diluted blood = C__<br />

0.02<br />

= 50C<br />

<strong>The</strong> dilution is 1 in 200<br />

/ Cells in 1 cu.mm. undiluted blood = 50 x 200C<br />

= 10,000C<br />

Precautions:<br />

…………………………………………………………………………………………………<br />

…………………………………………………………………………………………………<br />

…………………………………………………………………………………………………<br />

…………………………………………………………………………………………………<br />

…………………………………………………………………………………………………<br />

Observations:<br />

1<br />

2<br />

3<br />

4<br />

5<br />

6<br />

7<br />

8<br />

9<br />

10<br />

11<br />

12<br />

13<br />

14<br />

15<br />

16<br />

17<br />

Name [Males]<br />

WBC Count<br />

(cells/cu.mm)<br />

Name[Females]<br />

WBC Count<br />

(cells/cu.mm)<br />

Blood<br />

Page | 32

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