Lab Manual - eScience Labs

Lab 9: Enzymes
5. Make a serial diluon of yeast soluon by adding 10mL yeast soluon from Beaker A and transfer
to Beaker B. Add 90 mL warm water (30-35°C) to Beaker B. Mix well by pipeng.
6. Take 10mL yeast soluon from Beaker B and transfer to Beaker C. Add 90 mL warm water (30-
35°C) to Beaker C. Mix well by pipeng.
7. Into the first test tube, add 5mL from Beaker A.
8. Quickly aach a balloon to the top of the test tube so that it will fill with the oxygen produced
by the enzyme reacon. It is important to execute this step quickly so that every bit of gas produced
will be captured.
9. Repeat this procedure by transferring 5ml from Beaker B into test tube 2, aaching the balloon,
and transferring 5 mL from Beaker C into test tube 3 and immediately placing the balloon
on top.
10. Swirl each tube to mix, and wait 30 seconds.
11. Wrap the string around the center of the balloon to measure the circumference. Measure the
length of string with a ruler. Record measurements in Table 1 below.
12. Repeat step 11 for the remaining balloons.
Table 1: Effect of Enzyme Concentraon on the Producon of Gas
Tube Amount of yeast Balloon circumference (cm)
1 0.05g
2 0.005g
3 0.0005g
Quesons
1. What is the enzyme in this experiment? What is the substrate?
2. Did you noce a difference in the rate of reacon in the tubes with different concentraons of
enzymes? Why or why not?
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