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Lab Manual - eScience Labs

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<strong>Lab</strong> 14: Mendelian Genecs<br />

The law of segregaon states that during<br />

meiosis, homologous (paired) chromosomes<br />

split (Figure 1). The law of independent<br />

assortment states that during<br />

meiosis, each homologous chromosome<br />

has an equal chance of ending up in either<br />

gamete, and alleles for individual<br />

genes segregate with the chromosomes<br />

on which they are located (Figure 2).<br />

Using corn as an example (Figure 2):<br />

Figure 2: Law of Independent Assortment<br />

• The large chromosome has the gene for kernel color (Y = yellow, y = blue).<br />

• The small chromosome has the gene for kernel texture (S = smooth (green); s = wrinkled (red)).<br />

When a dominant allele is present, that characterisc is expressed, regardless of the second allele. In<br />

this case both the Yy and YY offspring will be yellow.<br />

A recessive allele is only expressed when both alleles are recessive. In this case only the yy combina-<br />

on is blue. The dominant allele is always represented by capital leers, while the recessive is represented<br />

by lower case leers.<br />

Genotype refers to the combinaon of alleles for a parcular trait. Phenotype refers to the appearance<br />

of that combinaon of alleles. In our example, the genotype of the diploid cell is Yy, Ss, while the<br />

phenotype is Yellow and Smooth.<br />

Figure 3: Monohybrid Cross<br />

Punne Square F 1<br />

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