Lab Manual - eScience Labs
Lab Manual - eScience Labs
Lab Manual - eScience Labs
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<strong>Lab</strong> 8: Respiraon<br />
Experiment 1: Fermentaon by Yeast<br />
Yeast cells produce ethanol and CO 2 during fermentaon. We will measure the producon of CO 2 to<br />
determine the rate of anaerobic respiraon in the presence of different carbohydrates.<br />
Note: Sucrose (a disaccharide) is made up of glucose and fructose. Glucose is a monosaccharide.<br />
Materials<br />
5 Respirometers (Figure 2)<br />
1% Glucose soluon<br />
1% Sucrose soluon<br />
Equal, Splenda, and sugar<br />
packets<br />
1 Yeast packet<br />
4 250mL Beakers<br />
100mL Graduated cylinder<br />
Warm water*<br />
Pipees<br />
Watch or mer*<br />
Permanent marker<br />
Ruler<br />
Measuring spoon<br />
*You must provide<br />
Figure 2: To make a respirometer, obtain two test tubes<br />
that fit into each other – one small plasc test tube and<br />
one large glass test tube for each respirometer.<br />
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