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Lab Manual - eScience Labs

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<strong>Lab</strong> 9: Enzymes<br />

2. Spray a paper towel with starch. Let dry for 2 hours.<br />

3. In the mean me, set up a control for this experiment. (Hint: What happens when IKI soluon is<br />

mixed with starch? What happens when it is mixed with another liquid?)<br />

4. Cut the food specimens so that a fresh surface is exposed.<br />

5. Gently rub each specimen on the paper towel, back and forth 10-15 mes. <strong>Lab</strong>el where each<br />

specimen was rubbed on the paper towel with a permanent marker.<br />

6. Rub a drop of saliva into the paper towel. (We know saliva contains amylase.)<br />

7. Wait 5 minutes.<br />

8. Hold the spray bole 10-12 inches from the paper towel, and mist with the IKI soluon spray.<br />

9. Observe where color develops, indicang the presence of starch.<br />

Quesons<br />

1. What is the funcon of amylase? What does amylase do to starch?<br />

2. What were your controls for this experiment? What did they prove?<br />

3. Why did you wait 5 minutes before spraying with IKI soluon?<br />

4. Which of the foods that you tested contained amylase? Which did not? What experimental<br />

evidence supports your claim?<br />

104

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