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Lab Manual - eScience Labs

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<strong>Lab</strong> 14: Mendelian Genecs<br />

c) What is your predicted rao of genotypes? Hint: think back to our example dihybrid<br />

cross<br />

• Repeat this process 4 mes (for a total of 5 trials).<br />

d) How similar are the observed phenotypes in each replicate?<br />

e) How similar are they if you pool your data from each of the 5 replicates?<br />

f) Is it closer or further from your predicon?<br />

g) Did the results from the monohybrid or dihybrid cross most closely match your<br />

predicted rao of phenotypes?<br />

h) Based on these results; what would you expect if you were looking at a cross of<br />

5, 10, 20 independently sorted genes?<br />

i) Why is it so expensive to produce a hybrid plant seed?<br />

j) In certain bacteria, an oval shape (S) is dominant over round and thick cell<br />

walls (T) are dominant over thin. Show a cross between a heterozygous oval,<br />

thick cell walled bacteria with a round, thin cell walled bacteria. What are the<br />

phenotype of the F 1 and F 2 offspring?<br />

5. The law of independent assortment allows for genec recombinaon. The following equaon<br />

can be used to determine the total number of possible genotype combinaons for any parcular<br />

number of genes:<br />

2 g = Number of possible genotype combinaons (where “g” is the number of genes)<br />

1 gene: 2 1 = 2 genotypes<br />

2 genes: 2 2 = 4 genotypes<br />

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