19.06.2015 Views

Lab Manual - eScience Labs

Lab Manual - eScience Labs

Lab Manual - eScience Labs

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

<strong>Lab</strong> 14: Mendelian Genecs<br />

Attention! Do not pour the beakers together.<br />

• #1 contains beads that are either yellow or blue.<br />

• #2 contains beads that are either green or red.<br />

• Both contain approximately the same number of each colored bead.<br />

• These colors correspond to the following traits (remember that Y/y is for kernel<br />

color and S/s is for smooth/wrinkled):<br />

• Yellow (Y) vs. Blue (y) Green (S) vs. Red (s).<br />

A. Monohybrid Cross: Randomly (without looking) take 2 beads out of #1.<br />

• This is the genotype of individual #1, record this informaon. Do not put these<br />

beads back into the beaker.<br />

• Repeat this for individual #2. These two genotypes are your parents for the next<br />

generaon. Set up a Punne square and determine the genotypes and phenotypes<br />

for this cross.<br />

• Repeat this process 4 mes (5 total). Put the beads back in their respecve beakers<br />

when finished.<br />

a) How much genotypic variaon do you find in the randomly picked parents of your<br />

crosses?<br />

b) How much in the offspring?<br />

c) How much phenotypic variaon?<br />

d) Is the rao of observed phenotypes the same as the rao of predicted phenotypes?<br />

Why or why not?<br />

e) Pool all of the offspring from your 5 replicates. How much phenotypic variaon do<br />

156

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!