Medical Mycology – Yeast Research | Head: Teresa GonçalvesObjectives1. With the final purpose <strong>of</strong> unravel the resistance<strong>of</strong> C<strong>and</strong>ida albicans to macrophage attack a studywas initiated with the aim to study the role <strong>of</strong>extracellular purines since ATP <strong>and</strong> its metabolite,adenosine, have been implicated in the immuneinflammatoryresponse as STOP signals.2. The increased number <strong>of</strong> yeast infectionsprompted us to study the epidemiology <strong>of</strong> yeastinfections in the Portuguese population attending aCentral Hospital.3. Our recent finding that the yeast Saccharomycescerevisiae responds to bacterial endotoxinactivating the HOG Signalling Pathway [J BiolChem 281: 24687‐24694(2006)] prompted us tostudy the impact <strong>of</strong> such activation in yeastmetabolism.4. With the identification in the dematiaceousfungus Alternaria infectoria <strong>of</strong> AiFKS <strong>and</strong> AiRHO,together with casp<strong>of</strong>ungin susceptibility, took us tostudy the combined effect <strong>of</strong> anti‐fungal cell wallinhibitors in collaboration with Pr<strong>of</strong> Neil Gow <strong>of</strong>the Institute <strong>of</strong> Medical Sciences, University <strong>of</strong>Aberdeeen, UK (manuscripts under preparation).Main Achievements1. Yeast metabolic response to the presence <strong>of</strong>bacterial endotoxin (one paper submitted)2. Combined effect <strong>of</strong> anti‐fungal cell wallinhibitors in A. infectoria: identification <strong>and</strong>cloning <strong>of</strong> the AiFKS gene <strong>and</strong> its regulatorAiRHO; casp<strong>of</strong>ungin susceptibility (2 papers underpreparation)3. Clinical mycology: a pathogenic yeast collectionwith 1000 isolates identified <strong>and</strong> characterisedusing molecular biology; an epidemiological study;a new diagnostic technology is being patented4. An in infection model using macrophage cellsinfected with C<strong>and</strong>ida albicans is currently beingused to study:a. the effect <strong>of</strong> purines <strong>and</strong> purinergic receptors inC. albicans clearance, indicating that adenosinemight affect this process trough specific adenosinereceptors (ongoing work)b. together with the Faculty <strong>of</strong> Medicine <strong>of</strong> Porto astudy was finished indicating that CUG ambiguityin C. albicans affects the phagocytic efficiency (onepaper under preparation)62Fig.1. a. C. albicans cell ingested by RAW264.7 macrophageb.,c. Yeast cells evading macrophage celld. Yeast cells inside the macrophage, <strong>for</strong>ming germ tubee. Yeast cell destroyed by phagolysosome
PublicationsAlarico S, da Costa MS, Empadinhas N (<strong>2008</strong>) Molecular <strong>and</strong> physiological role <strong>of</strong> the trehalosehydrolyzingalpha‐glucosidase from Thermus thermophilus HB27. J. Bacteriol. 190(7):2298‐305.Albuquerque L, Rainey FA, Nobre MF, da Costa MS (<strong>2008</strong>) Elioraea tepidiphila gen. nov., sp. nov., a slightlythermophilic member <strong>of</strong> the Alphaproteobacteria. Int. J. Syst. Evol. Microbiol. 58(Pt 4):773‐8.Albuquerque L, Tiago I, Taborda M, Nobre MF, Veríssimo A, da Costa MS (<strong>2008</strong>) Bacillus isabeliae sp. nov.,a halophilic bacterium isolated from a sea salt evaporation pond. Int. J. Syst. Evol. Microbiol. 58(Pt 1):226‐30.Alves M, Nogueira C, de Magalhães‐Santʹana A, Chung AP, Morais PV, da Costa MS (<strong>2008</strong>)Nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface <strong>of</strong> bottles <strong>of</strong> saline solution used inwound cleansing. Int. J. Syst. Evol. Microbiol. 58(Pt 12):2939‐44.Antunes A, Rainey FA, Wanner G, Taborda M, Pätzold J, Nobre MF, da Costa MS, Huber R (<strong>2008</strong>) A newlineage <strong>of</strong> halophilic, wall‐less, contractile bacteria from a brine‐filled deep <strong>of</strong> the Red Sea. J. Bacteriol.190(10):3580‐7.Antunes A, Taborda M, Huber R, Moissl C, Nobre MF, da Costa MS (<strong>2008</strong>) Halorhabdus tiamatea sp. nov., anon‐pigmented, extremely halophilic archaeon from a deep‐sea, hypersaline anoxic basin <strong>of</strong> the Red Sea,<strong>and</strong> emended description <strong>of</strong> the genus Halorhabdus. Int. J. Syst. Evol. Microbiol. 58(Pt 1):215‐20.Callegan RP, Nobre MF, McTernan PM, Battista JR, Navarro‐González R, McKay CP, da Costa MS, RaineyFA (<strong>2008</strong>) Description <strong>of</strong> four novel psychrophilic, ionizing radiation‐sensitive Deinococcus species fromalpine environments. Int. J. Syst. Evol. Microbiol. 58(Pt 5):1252‐8.Cava F, Zafra O, da Costa MS, Berenguer J (<strong>2008</strong>) The role <strong>of</strong> the nitrate respiration element <strong>of</strong> Thermusthermophilus in the control <strong>and</strong> activity <strong>of</strong> the denitrification apparatus. Environ. Microbiol. 10(2):522‐33.Empadinhas N, Albuquerque L, Mendes V, Macedo‐Ribeiro S, da Costa MS (<strong>2008</strong>) Identification <strong>of</strong> themycobacterial glucosyl‐3‐phosphoglycerate synthase. FEMS Microbiol. Lett. 280(2):195‐202.63Empadinhas N, da Costa MS. <strong>2008</strong>. Osmoadaptation mechanisms in prokaryotes: distribution <strong>of</strong>compatible solutes. Int Microbiol. 11(3):151‐61.Empadinhas N, da Costa MS (<strong>2008</strong>) To be or not to be a compatible solute: bioversatility <strong>of</strong>mannosylglycerate <strong>and</strong> glucosylglycerate. Syst. Appl. Microbiol. 31(3):159‐68.Hipolito E, Faria E, Alves AF, de Hoog GS, Anjos J, Gonçalves T, Morais PV, Estevão H (<strong>2008</strong>) Alternariainfectoria brain abscess in a child with chronic granulomatous disease. Eur. J. Clin Microbiol Infect. Dis. 28:377‐380.Nobre A, Alarico S, Fern<strong>and</strong>es C, Empadinhas N, da Costa MS (<strong>2008</strong>) A unique combination <strong>of</strong> geneticsystems <strong>for</strong> the synthesis <strong>of</strong> trehalose in Rubrobacter xylanophilus: properties <strong>of</strong> a rare actinobacterialTreT. J. Bacteriol. 190(24):7939‐46.Paulo C, Mourão C, Veiga PM, Marques JM, Rocha G, Alves AF; Querol A, Meliço‐Silvestre AA,Gonçalves I, Flores, ClementeC, Gonçalves T (<strong>2008</strong>) Retrospective analysis <strong>of</strong> clinical yeast isolates in ahospital in the centre <strong>of</strong> Portugal: spectrum <strong>and</strong> revision <strong>of</strong> the identification procedures. Med. Mycol. DOI:10.1080/13693780802709081.Pereira PJ, Empadinhas N, Albuquerque L, Sá‐Moura B, da Costa MS, Macedo‐Ribeiro S (<strong>2008</strong>)Mycobacterium tuberculosis glucosyl‐3‐phosphoglycerate synthase: structure <strong>of</strong> a key enzyme inmethylglucose lipopolysaccharide biosynthesis. PLoS ONE 3(11):e3748.Sá‐Moura B, Albuquerque L, Empadinhas N, da Costa MS, Pereira PJ, Macedo‐Ribeiro S (<strong>2008</strong>)Crystallization <strong>and</strong> preliminary crystallographic analysis <strong>of</strong> mannosyl‐3‐phosphoglycerate synthasefromRubrobacter xylanophilus. Acta Crystallogr. Sec.t F. Struct. Biol. Cryst. Commun. 1;64(Pt 8):760‐3.
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