areas as the gills, fins, and eyes. Other observations <strong>of</strong> the general physicalhealth <strong>of</strong> the specimens may be made as necessary (removal <strong>of</strong> a scale orspine for aging, determination <strong>of</strong> sex, and observations <strong>of</strong> overall health suchas parasites, fin erosion, skin lesions). If field dissections <strong>of</strong> captured fish areconducted, observations <strong>of</strong> the internal organs, such as liver, muscles, andurogenital organs, should be made to assess the presence <strong>of</strong> any grossabnormalities. Additionally, field observations <strong>of</strong> behavioral changes (e.g.,twitching, gasping, long-axis whirling, and convulsions) should be made.If site COPECs are known to be associated with histopathological effects onfish, a subset <strong>of</strong> the fish collected should be subjected to internalhistopathological analysis (e.g., this analysis serves as a measurementendpoint associated with the assessment endpoints <strong>of</strong> survival, growth,development, reproduction, and maintenance <strong>of</strong> healthy fish populations).Laboratory methods for histopathological evaluation can be found in EPA(1995c) and Schmitt and Dethl<strong>of</strong>f (2000).Biological sampling for primary producers, such as benthic algae andplankton, may be needed for specialized evaluations such as a food web study.Refer to Appendix B for detailed procedures.6.2.1.4 Surface Water Toxicity TestsWhen surface water analytical data exceed the fresh water or saline watercriteria listed in the SWQS (N.J.A.C. 7:9-B) or the NJDEP ESC Table,surface water toxicity tests can provide an indication <strong>of</strong> potential effects onaquatic biota.Populations <strong>of</strong> aquatic organisms (e.g., fish, invertebrates, and plants) can beimpacted when the quality <strong>of</strong> the water in which they live is changed. Themagnitude <strong>of</strong> the impact depends on the magnitude <strong>of</strong> the change to eitherphysical parameters (e.g., temperature, DO, pH, suspended solids, andsalinity) or chemical parameters (e.g., concentrations <strong>of</strong> salts, nutrients, orchemicals). Aquatic toxicity testing is used to measure the effects <strong>of</strong> thesechanges on aquatic organism survival, growth, or reproduction using astandardized suite <strong>of</strong> laboratory organisms (e.g., fathead minnows or daphniafor freshwater; sheepshead minnows or mysid shrimp for saline water),following standardized testing protocols.Surface water toxicity tests should follow established USEPA guidance foraqueous toxicity tests. Acute toxicity studies should be performed inaccordance with Methods for Measuring the Acute Toxicity <strong>of</strong> Effluents andReceiving Waters to Freshwater and Marine Organisms (USEPA, 2002b).Short-term chronic toxicity studies should be performed in accordance withShort-Term Methods for Estimating the Chronic Toxicity <strong>of</strong> Effluents andReceiving Waters to Freshwater Organisms (USEPA, 2002c).Laboratories conducting surface water toxicity tests are required via N.J.A.C.7:26E-2.1(a)1i to be certified for "Category WPP08 Toxicity Testing" underN.J.A.C. 7:18.<strong>Ecological</strong> <strong>Evaluation</strong> <strong>Technical</strong> <strong>Guidance</strong> Document 53Version 1.2 8/29/12
Details regarding surface water toxicity test procedures can be found inAppendix C.6.2.2 SedimentsBasic sediment sampling guidance is provided in Section 5.3. The followingsections describe data development issues and the suggested protocols to be usedin more comprehensive sediment sampling programs.6.2.2.1 Sampling Plan Design for Study and Reference AreasThe investigator is referred to N.J.A.C. 7:26E-2.1 for appropriate analyticalprotocol and quality assurance requirements. The investigator is also referredto Section 10.0 for additional guidance on QA/QC and the preparation <strong>of</strong>quality assurance plans.Developing an appropriate sediment sampling plan is a critical step insediment assessment and monitoring studies. Sample location selection andsampling methods will result from the study design. A properly designedstudy will control extraneous sources <strong>of</strong> variability or error and allow for datathat are representative <strong>of</strong> the sediment and fulfill the study objectives.A primary goal <strong>of</strong> a sediment investigation is to determine whether thepresence <strong>of</strong> COPECs in sediment is adversely affecting sediment-dwellingorganisms. In the case <strong>of</strong> bioaccumulative compounds or constituents, theprimary goal is to determine whether these constituents are accumulating inthe tissues <strong>of</strong> aquatic organisms to such an extent that they pose a hazard tosediment-dwelling organisms and aquatic-dependent wildlife.A comprehensive sediment investigation should result in the following:Identification and quantification <strong>of</strong> the contaminants present in sediment;Understanding <strong>of</strong> the vertical and horizontal distribution <strong>of</strong> the COPECs inthe sediment relative to the appropriate ESCs or background contaminantlevels;Understanding <strong>of</strong> the physical, chemical, and biological processes andtemporal trends affecting the fate and bioavailability <strong>of</strong> the sedimentCOPECs at the site;Identification <strong>of</strong> the complete exposure pathways for sedimentcontamination;Identification <strong>of</strong> current potential ecological risks posed by the sedimentcontamination;Identification <strong>of</strong> potential bioaccumulation risks; andUnderstanding <strong>of</strong> the impact <strong>of</strong> disturbance <strong>of</strong> impacted sediment on thespecies in and around the site that are dependent on the aquatic system.I. <strong>Evaluation</strong> <strong>of</strong> Existing Site Investigation DataThe first step in designing a sediment investigation involves evaluating thedata and site-specific information collected as part <strong>of</strong> the site characterizationduring the SI. For sediment investigations, the following information shouldbe evaluated before developing a sediment study design:<strong>Ecological</strong> <strong>Evaluation</strong> <strong>Technical</strong> <strong>Guidance</strong> Document 54Version 1.2 8/29/12
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Ecological EvaluationTechnical Guid
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Appendix B - Sampling Procedures fo
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Appendix C - Surface Water Toxicity
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Short-term chronic studies, endpoin
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Appendix D - Sediment Toxicity Test
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Toxicity Test DesignSediment toxici
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Appendix E - Sediment Pore Water an
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The seven-day daphnid survival and
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esults are then evaluated using USE
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Surber or Square-foot BottomThis sa
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Appendix H - Soil Toxicity TestingS
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another sample may still have a sub
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conservative approach from an ecolo
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Data PresentationTabular presentati