VACCINE

“A cell line (MDCK) of dog kidney origin grows
on a glass surface as a mosaic of epithelium
Science • January 1969
Secretory activity and oncogenicity
of a cell line (MDCK) derived from canine kidneyand
A cell line (MDCK) of dog kidney origin grows on a glass surface as a mosaic of epithelium
with many multicellular hemispherical vesicles. The cells lining the blisters actively
secrete into the cyst cavities. Suspensions of these cells injected intravenously in the
chick embryo produce brain metastases resembling adenocarcinoma.
[Editors Note: The MDCK (NBL-2) (ATCC® CCL-34) cell line has been used since
1958 to produce influenza and other vaccines]
http://www.sciencemag.org/content/163/3866/472.long
Information on the MDCK cell line
http://www.atcc.org/products/all/CCL-34.aspx
with many multicellular hemispherical vesicles.
The cells lining the blisters actively secrete into
the cyst cavities. Suspensions of these cells injected
intravenously in the chick embryo produce
brain metastases resembling adenocarcinoma.”
[In a departure from the use of traditional egg-based
vaccines the FDA approved Flucelvax for Novartis on
November 20th, 2012. Flucelvax was the first mammalian
cell-based influenza vaccine in the US. The Madin-Darby
canine kidney cell line is cultured to produce the vaccine.
The primary advantage of MDCK over traditional egg-based
manufacturing is rapid growth.]