VACCINE

Environmental Science And Pollution Research International • November 2014
Effects of aluminium and bacterial lipopolysaccharide
on oxidative stress and immune parameters
in roach, Rutilus rutilus L
Author information
Jolly S1, Jaffal A, Delahaut L, Palluel O,
Porcher JM, Geffard A, Sanchez W, Betoulle S.
Université de Reims Champagne-Ardenne
UMR-I02 SEBIO, BP 1039, 51687
Reims Cedex 2, France
sabrina.jolly@univ-reims.fr
Abstract
Aluminium is used in diverse anthropogenic processes at the origin of pollution
events in aquatic ecosystems. In the Champagne region (France), high concentrations
of aluminium (Al) are detected due to vine-growing practices. In fish, little is known
about the possible immune-related effects at relevant environmental concentrations.
The present study analyzes the simultaneous effects of aluminium and bacterial lipopolysaccharide
(LPS), alone and in combination, on toxicological biomarkers in the
freshwater fish species Rutilus rutilus. For this purpose, roach treated or not with LPS
were exposed to environmental concentrations of aluminium (100 μg/L) under laboratory-controlled
conditions for 2, 7, 14 and 21 days. After each exposure time, we
assessed hepatic lipoperoxidation, catalase activity, glutathione reductase activity and
total glutathione content. We also analyzed cellular components related to the LPSinduced
inflammatory response in possible target tissues, i.e. head kidney and spleen.
Our results revealed a significant prooxidant effect in the liver cells and head kidney
leukocytes of roach exposed to 100 μg of Al/L for 2 days. In liver, we observed more
lipoperoxidation products and lower endogenous antioxidant activity levels such as
glutathione reductase activity and total glutathione content. These prooxidant effects
were associated with a higher oxidative burst in head kidney leukocytes, and they
were all the more important in fish stimulated by LPS injection. These findings demonstrate
that environmental concentrations of Al induce oxidative and immunotoxic
effects in fish and are associated to an immunomodulatory process related to the inflammatory
response.
“These findings demonstrate that
environmental concentrations of Aluminum
induce oxidative and immunotoxic effects in fish
and are associated to an immunomodulatory
process related to the inflammatory response.”
http://www.ncbi.nlm.nih.gov/pubmed/24996940