NAMS 2002 Workshop - ICOM 2008

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esults provide important insights into the design and operation of virus filtration systems for the production of therapeutic proteins.
Biomedical and Biotechnology I – 2 Monday July 14, 10:15 AM-10:45 AM, Honolulu/Kahuku Developments in Membrane Affinity Chromatography for Monoclonal Antibody Recovery S. Dimartino, University of Bologna, Bologna, Italy C. Boi, University of Bologna, Bologna, Italy G. Sarti (Speaker), University of Bologna, Bologna, Italy, giulio.sarti@unibo.it The great number of process development for monoclonal antibodies, presently in development stage, has emphasized the capability limits of the biotech industry. The recent improvements of cultivation technology, allow also to achieve high titers of monoclonal antibody in cell supernatants, and the present bottleneck for MABs’s production is associated to the downstream process required for product recovery. Bead-based affinity chromatography with Protein A is widely used in the primary capture stage. Membrane affinity chromatography has not yet experienced extensive application due to the lower capacity of membrane supports compared to chromatographic beads, yet it has several advantages deserving serious attention. This work is focused on the purification of Immunoglobulin G (IgG) with affinity membranes. A new Protein A affinity membrane (Sartorius, Göettingen, Germany), as well as affinity membranes prepared with synthetic ligands have been characterized in detail in batch and dynamic experiments. The membranes have been analysed by using pure solutions of polyclonal IgG, to determine their binding capacity, as well as a cell supernatant containing monoclonal IgG, to investigate their selectivity and general behavior. The influence of process conditions like flow rate and feed concentration on adsorption and elution have been studied to obtain indications for the optimal process conditions. The affinity membrane purification process was also simulated with a mathematical model which was validated by using the experimental data obtained. The model can simulate adsorption, washing and elution steps by taking into account all the relevant transport phenomena.
Page 1 and 2: ICOM 2008 Oral Presentation Proceed
Page 3 and 4: Professor Yoram Cohen Professor Joh
Page 5 and 6: ICOM 2008 Staff: The University of
Page 7 and 8: ICOM 2008 Workshop Schedule: Saturd
Page 9 and 10: Monday, July 14 - Afternoon Session
Page 11 and 12: Tuesday, July 15 - Afternoon Sessio
Page 13 and 14: 8:15AM 8:35AM EMS Barrer Prize (Mau
Page 15 and 16: Friday, July 18 - Morning Sessions
Page 17 and 18: Oral Presentation Abstracts Morning
Page 19 and 20: Gas Separation I - 1 - Keynote Mond
Page 21 and 22: esulting permeability selectivity o
Page 23 and 24: chain packing. To ensure a well pha
Page 25 and 26: Gas Separation I - 5 Monday July 14
Page 27 and 28: satisfactory way the corresponding
Page 29 and 30: Drinking and Wastewater Application
Page 35 and 36: an adjusted water management in the
Page 37 and 38: prospect for new energy sources as
Page 39 and 40: oxygen removal to the desired pH. C
Page 41 and 42: (Cl - , SO4 2- , As(V)) and water t
Page 43 and 44: [1] R. Lima de Miranda, J. Kruse, K
Page 45 and 46: Polymeric Membranes I - 4 Monday Ju
Page 47 and 48: Polymeric Membranes I - 5 Monday Ju
Page 49: Biomedical and Biotechnology I - 1
Page 53 and 54: Acknowledgments The Authors acknowl
Page 55 and 56: isolation of CD34+ cells was achiev
Page 57 and 58: Biomedical and Biotechnology I - 6
Page 59 and 60: membranes were then challenged with
Page 61 and 62: in crossflow mode operation were in
Page 63 and 64: hydroxide. For Mg/Ca = 0, the fouli
Page 65 and 66: improvement of filterability also t
Page 67 and 68: scale where fouling rates are commo
Page 69 and 70: observed in two subsequence phenome
Page 71 and 72: Membrane Modeling I - Fundamental A
Page 73 and 74: Membrane Modeling I - Fundamental A
Page 75 and 76: of the impact of the operating para
Page 77 and 78: Oral Presentation Abstracts Afterno
Page 79 and 80: Hybrid and Novel Processes I - 2 Mo
Page 81 and 82: Arora, M.B., J.A. Hestekin, S.W. Sn
Page 83 and 84: Conclusions Reverse electrodialysis
Page 85 and 86: energy recovery. This is a remarkab
Page 87 and 88: eaction with mediator due to the co
Page 89 and 90: Nanofiltration and Reverse Osmosis
Page 91 and 92: 4.3x10 -6 to 7.4x10 -6 cm 2 /s. Bas
Page 93 and 94: The development of these membranes
Page 95 and 96: permeability, defined as water perm
Page 97 and 98: supports including charged (PSF/SPE
Page 99 and 100: [4] L. M. Robeson, Journal of Membr
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y conventional polymers and provide
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[3] P.M. Budd, K.J. Msayib, C.E. Ta
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Nanostructured Membranes I - 5 Mond
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Fuel Cells I - 1 Monday July 14, 2:
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investigated at first. As a result,
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Desalination I - 2 Monday July 14,
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Composite Polymeric Membrane Format
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NAMS Alan S. Michaels Award - 1a Tu
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NAMS Alan S. Michaels Award - 2 Tue
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opportunities for improving membran
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NAMS Alan S. Michaels Award - 4b Tu
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Nanofiltration and Reverse Osmosis
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[4] K. Vanherck et al. Accepted for
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accumulation had a strong effect on
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and, if so, to develop correlations
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non-porous and porous membranes are
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[2] Palmeri, J., Sandeaux, R. Sande
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fouling. Information obtained from
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This work performed under the auspi
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etween Ru(bi-pyridine)3 2+ and meth
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[1] M. Barboiu, C. Luca, C. Guizard
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Nanostructured Membranes II - 5 Tue
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Nanostructured Membranes II - 6 Tue
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than 10 nm. XRD data suggest that t
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Pervaporation and Vapor Permeation
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ethanol/butanol (non solvent) combi
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forward osmosis, the RO process is
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Osmotically Driven Membrane Process
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References: [1] Stupp, S. I.; Lebon
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temperatures might bring about the
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References [1] S. Benita, Microenca
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solvent, were investigated (where a
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ease of manipulation as this allows
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Horizontal shrinkage does not have
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Absorption of water was determined
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Gas Separation II - 1 - Keynote Tue
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Gas Separation II - 2 Tuesday July
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concentration. These results are co
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Gas Separation II - 5 Tuesday July
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will be shown that homogeneous film
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which can adsorb on the surface of
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Drinking and Wastewater Application
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were collected, the viable bacteria
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pressure, and permporosimetry with
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Inorganic Membranes I - 3 Tuesday J
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Membrane Fouling - UF & Water Treat
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Membrane Modeling II - Gas Separati
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[10] Wang BG, Lv HL, Yang JC. Chem
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Membrane and Surface Modification I
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Oral Presentation Abstracts Morning
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Gas Separation III - 1 - Keynote We
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Gas Separation III - 2 Wednesday Ju
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separation was next blended with di
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Polymeric Membranes II - 1 - Keynot
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Polymeric Membranes II - 3 Wednesda
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5. Erdodi, G.; Kennedy, J. P.; Prog
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observed for different locations in
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Biomedical and Biotechnology II - 3
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applied in the first two fields wil
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distribution of SBMA units within t
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5500 ppm (15 mM) was lowered to 30
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increasing the effective size of th
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etween 0 and 1), the automatic feed
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Membrane Modeling III - Process Sim
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Membrane Modeling III - Process Sim
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models developed suggests that ther
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start level has a huge impact on th
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Ultra- and Microfiltration I - Tran
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all three binary protein UF systems
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Synthetic solutions of ±-lactalbum
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Oral Presentation Abstracts Morning
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Gas Separation IV - 2 Thursday July
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6. T. C. Merkel, Z. He, I. Pinnau,
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EMS Barrer Prize - 1b Thursday July
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EMS Barrer Prize - 3 Thursday July
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EMS Barrer Prize - 4b Thursday July
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EMS Barrer Prize - 5 Thursday July
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therapy options have been modelled
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Ultra- and Microfiltration II - Pro
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in the vicinity of the rising bubbl
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of feed water ionic strength) on re
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technology that has gained growing
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Optimize polymeric membranes for sa
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Inorganic Membranes II - 2 Thursday
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confirms that the carbon dioxide pe
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permeability, stability issues comp
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Fuel Cells II - 2 Thursday July 17,
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5. Conclusion In this work, the evo
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Oral Presentation Abstracts Afterno
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an essentially pure H2 product is c
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In the presentation, we will presen
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was observed, indicative of the sim
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References Arora, M.B., J.A. Hestek
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performance of fibers is analyzed a
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Membrane Fouling III - RO & Biofoul
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[4] H. Noureddini, Book of Abstract
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component (PX, OX) separation via p
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Generally these results were in goo
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introduction of methyl groups into
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separation quality (10 µS/cm) and
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Desalination II - 3 Thursday July 1
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drop are linearly related (although
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antiscalant oxidation has been limi
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oth 15±5 kDa, which suggests that
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Atomic Force Microscopy (AFM), and
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y single gas permeation experiments
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PDMS toplayers were only partially
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noting that cross-linking agents co
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such extended parameter space in th
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Hybrid Membranes - 6 Thursday July
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Plenary Lecture III Friday July 18.
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Gas Separation V - 1 - Keynote Frid
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References: [1] H. Lin, E. Van Wagn
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elative contributions of Fickian di
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Gas Separation V - 4 Friday July 18
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Gas Separation V - 6 Friday July 18
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Electron Microscopy (TEM) analyses
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- Hybrid RO train: A hybrid RO trai
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concentration as well as on the sod
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scaling deposits in the DCMD device
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Silica colloidal solution with diff
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Membrane Fouling IV - RO & Desalina
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Polyacrylonitrile (PAN) UF membrane
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the polymer dope was PEI (12 wt%),
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Inorganic Membranes III - 1 - Keyno
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Inorganic Membranes III - 2 Friday
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CO2/H2 selectivity at high temperat
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ammonia complexes in aqueous soluti
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possible to have a membrane capable
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fired boiler flue gas, SOx and merc
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EPR/Ago/p-benzoquinone composite sh
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elated to the presence of free elec
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surroundings. When the temperature
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that no absorbent was detected in t
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days of filtration from 120 to 7-10
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was performed to restore membrane f
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with the SRT of 65 days, no correla
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Results Critical flux measurements
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MLSS is not directly proportional t
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Fuel Cells III - 2 Friday July 18,
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Fuel Cells III - 4 Friday July 18,
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Ultra- and Microfiltration III - Me
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can be deduced from the imaginary p
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membranes. The analysis of the ligh
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Membrane Contactors - 2 Friday July
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absorption liquid in the pores of t
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Packaging and Barrier Materials - 1
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Historically, all the approaches de
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