NAMS 2002 Workshop - ICOM 2008

eaction with mediator due to the conductivity of the support. Various enzyme
immobilisation techniques on porous supports have been developed [3] . On the
other hand, the elaboration of a matrix polymer based on polypyrrole obtained by
electrochemistry is a manufacturing technique, well mastered in the IEM [4] to
allow for producing stable conductive interfaces. At the cathode, oxygen is
directly reduced to water by laccase or BOD and at the anode glucose is oxidised
in gluconolactone by glucose oxidase, in the presence of their respective redox
mediators 2,2-azinobis(3- ethylbenzothiazoline-6-sulfonate) and 8-
hydroxyquinoline-5-sulfonic acid. The enzyme/mediator couples were
immobilized by covalent linkage via an N-substituted polypyrrole matrix
beforehand electrodeposited on carbon porous electrodes.
Experiments were conducted to determine the activity and the stability of the
enzymes immobilized on the electrocatalytic membrane. Operational conditions
and performances of the electrocatalytic membrane have been studied by
electrochemistry. These electrochemical studies will be carried out in model
conditions [5,6] in a physiological environment. The feasibility of each enzyme
contactors was demonstrated by chronoamperometry and current voltage
measurements using electrochemical halfs cells. Performances of the glucose/O2
biofuel cell were demonstrated by current voltage curves operating at variable
external loads.
The electrocatalytic membrane presented good and stable current densities that
established the feasibility of the co- immobilization of both enzyme and its
mediator on the electropolymerized films and of an operative glucose/O2 biofuel
cell.
1. G. Tayhas, R. Palmore, H.H. Kim, J. Electroanal. Chem. 1999, 464, 110
2. Kendall K., Nature Materials, 2002, 1, 211
3. G. Merle, L. Brunel, S. Tingry, M. Cretin, M. Rolland, K. Servat, C. Jolivalt, C. Innocent, P.
Seta, Mat. Sci. Eng C. (in press)
4. A.Naji, C. Marzin, G. Tarrago, M. Cretin, C. Innocent, M. Persin, J. Sarrazin, J. Applied
Electrochem. 31, 2001, 547-557
5. K. Servat, S. Tingry, L. Brunel, S. Querelle, M. Cretin, C. Innocent, C. Jolivalt and M. Rolland,
J. Appl. Electrochem. 37, 23007, 121
6. L. Brunel, J. Denele, K. Servat, K.B. Kokoh, C. Jolivalt, C.Innocent, M Cretin, M. Rolland and
S. Tingry, Electrochem. Comm. 9, 2007, 331