L./f*A tuf ac.-lL,;l u sV})

3,9,1.3. HDL,CtotBr.,!l (,ngdt)
IIDl{hot€slerot ws dct min.d by $ing ! @no.rcht dr.gnosjc kn (Hoda
' c8r, No.H 8206), plsmo (2O0pD and ditul.n E.g.nt (50oft) weE pllccd in rhe t$r
lube, u vos mixql we ond o ower b rhnd tor rO nrinurer { Mh r€mpe.!r!re. Th€n ir
wrs eDrifu8ed tor l0 nrinurs ar 4OOO enr, A0er c.ntifugalior ?0 !lsupernaLhr wd
laren in anoth€r resr rube. chotesrqol wolking rq8enr (2mt) wq udded. Thc btlnk 6
daft by t king 20Ft distilei wakr and 23mt chol.slcrct Mgenl whitettie sl,nd&l was
made by adding 2qrt choleskrot ,toidord n, xnt clDtc{erot r€age,r. Tlrre r@
nEubared ar rooD tehremru.c l.o. 5 mirures. ,l.hc lblorbdna ot r*,own ,nd shied
blank resgenr ar 5Oi nN was obsc.vc!. tlDt,-CholesGrot w6 cotcutor€d using rhe
Absodqncc of utrkrowr
HDL-Chol_ (hs/dt) = -_.._-__.'-_--.____ x
A bsorbo.c. o I sr.ndrrd
co.c of s|andod = 2oo,rgldt)
3.9.1.4. LDL ctbtqt.rct (hC/rI)
LDLcholsr.ot ws Etcltcr.d usinS rhe ao owi,g
LDl-cholsrdot (nrs/dt) = Torat Chot$lerot - I tDL Cnobsreol - Trievlatrcml;
J.9.1.5. Triecytgtyemb (ng/dD
The riacytglycerols w.a deGnnitr.ii by lsing a,nd.rcEt di.Fosric kn
(Fluirest). l0 !l sradsd lnd somptc cacl was ld.n inb resr rub€s qi$ on€ bletc
I000ul ioAing Rged ios ,Jd.o ro.d!h u rdb.s ond nired nopert,. Te$ rubs
weE incubated fo. 5 hinut6 0r lToC tiJr 5 mhrubs aM ab$rhance ks nesu€d
aSain! blul reagcnr or (O0nm.
lli.cylglyceo h were calcu l.red by th€ to|owitr g exprc$ion :
rr6c)tstyc0ok ( irgdD -
3.9.1,6. Tokl Droroi,, (g/dD
-
Con@nrBrion oI s6d.rd
------"---- r ConcEnk rion ot. sbndard.
Th. pl.sna DbGin @ d.tchirEd by usir8 olorih.|rio m€thod(sio-Rlys
CATrrl4oJ),2,5 mt ofbiurcl rcrgc was $kcn in dl€ lsbeted lesr rubd.0.05 nt of
t