L./f*A tuf ac.-lL,;l u sV})

3.9.2 Phsus trnzymcs
ctur@are prruv.re TEnMina. rCpD ( L/n,t) tud Ctarm.k Ox!to@hlu
TmNaninas (CoT ) (U/nD sor€ decnnhd aner 4 .od s e.k!
3.9.2.1 Ctui.m.r€ pyruvrte ar.ri.bi!$e (cpT U/Dr)
n'is vas deranii|ed by u!i[s rbe Rsilnmn_|rdntet cobnn.ric m.lhod hy u,ins
Quinrics Ctinioa Aptic.do (ecA) kii
Di5ut,.J war.r rtoooltj *a! pted ,n 6 n.nd6rd tubB Md ,Oo. ajo,,O0. Irq
25ql CPT tubsrmtes inluh. t, ?, 3,4,5 lmt 6 reE oddqt qperivcty- Tho pippd.d
50, 100, 150,200 ond 2toptstandard in $ese lcsftube, Adde.] ,O*, ,_""1*r"",
lo eeh tubs. l lEy w€ft ,nired dd olowed lo jrrnd ar tuD @mp€runrE tor zo naod*.
Fndly 5nl of Naott (0.4N) ws sdden. Absrban@ was @rdod ,ritr l j Dinures
!8.irsl a warer btaht {l 505hn. Usirg concenlmror ard loeroac. . sludud tacror
wrs catcuhred,
cl'T rubrtnr! (50011) vft pl!.M ln m$1. lah !0d tlouhLd || !7,C tor,
nlnut ! ard dd.d 100 !l '.|nptc b il. Ad&d 50OIt c.lour dev.togff ,nd t.r lr ro arrd
lor 20 nrio. al MD ledpcialuE_ tmt ofNaOI I (0.4a, Ms .ddal nd cltowed b ltlndrd
lor 15 nin. r{ room lmpedure,-l.hc rrehnlane w6 norcd atr.r t5 hirlts.gaiNl
w.r.! btdk.i 505nn. For opT &riviry valu€, ri..bsorbea o6loin"a * ini"goto.a
3.9.2.2. Ctrt.m.re o!.to.ebr. t..6mto.* ICOT U/or:
Clu|lmdc O\sloaetore tEnlominss. iCOT] Ms dereimrned b) R.irm.a
Frok.l oolo.in€ric mertD! by (eCA) kii
l000rt of,lisu ot w!k. v6 tdtq in o nendard lub6.|ld i00,450,400.350,
250t1 COT $bsr.a|c in lrb. 1,2,3,4,j ed 6 *cE add€d, Bpa(t|y. rhd pip.t& 50,
I0O, rJ0. 200.nd 2ro,rr sr&d!^l h lh*. rub.j Added 5OOft orcr_, *U_, ," "".n
tob.. Thes w.F mixed und . ow.d ro sund at rem &mpsoruE ror 20 nanu16,
FiMlly a