Fütterungsbedingte mikrobielle Zusammensetzung von Rinderkot ...

4.3 Results ............................................................................................................... 18
4.3.1. Extractant efficiency, variability, and stability of the CFE method ........ 18
4.3.2. Microbial biomass and ergosterol in incubated faeces........................... 20
4.3.3. Relationships of microbial indices in faeces of different heifers............. 21
4.4 Discussion.......................................................................................................... 22
4.4.1. Microbial biomass in cattle faeces by the CFE method .......................... 22
4.4.2. Bacterial and fungal distribution in cattle faeces ................................... 24
4.4.3. Conclusions ............................................................................................. 26
4.5 References ......................................................................................................... 27
5. Microbial biomass in faeces of dairy cows affected by a nitrogen deficient diet ... 35
5.1 Introduction ....................................................................................................... 36
5.2 Materials and Methods ...................................................................................... 38
5.2.1. Feeding regime ........................................................................................ 38
5.2.2. Faecal C and N fractions ........................................................................ 39
5.2.3. Microbial biomass C and N..................................................................... 40
5.2.4. Ergosterol analysis .................................................................................. 40
5.2.5. Amino sugars ........................................................................................... 41
5.2.6. Statistical analysis ................................................................................... 42
5.3 Results ............................................................................................................... 42
5.4 Discussion ......................................................................................................... 47
5.4.1. Microbial indices ..................................................................................... 47
5.4.2. Bacterial and fungal contribution to microbial tissue ............................ 48
5.4.3. Digestibility affected by N deficient diet ................................................. 50
5.5 Conclusions ........................................................................................................ 51
5.6 References .......................................................................................................... 52
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