Fütterungsbedingte mikrobielle Zusammensetzung von Rinderkot ...

4. Determination of microbial biomass and fungal and bacterial distribution in cattle faeces 15
such as acid detergent lignin, acid detergent fibre, neutral detergent fibre, and crude
fibre were determined by near-infrared spectroscopy (FOSS 6500, Rellingen, Germany)
as described by Papke and Sundrum (personal communication) after appropriate
calibration and validation. Table 1 shows mean values derived from five different
heifers, each analysed in triplicate. Mean organic C varied around 428 mg g -1 dry
weight (DW) with a C/N ratio of 16. The coefficient of variation (CV) between the three
analytical replicates and five heifers was usually below 5%. An exception was the NH4 +
content between the five heifers.
Tabelle 2 (Table 1): Variability of pH, elemental composition and organic components in cattle faeces from five identically fed heifers
Table 1 Variability of pH, elemental composition and organic components in
cattle faeces from five identically fed heifers
Mean heifer CV replicate (%) CV heifer (±%)
pH (H2O) 8.3 0.69 2.9
(mg g -1 DW)
C 428 0.75 1.4
N 22 0.19 3.0
NH4 + 1.4 ≤ 0.3 14
C/N 16 0.77 4.2
P 8.5 2.1 2.1
S 3.5 2.5 2.5
Na 1.0 3.6 3.6
K 5.8 2.1 2.1
Ca 22 2.6 2.6
Mg 8.0 2.6 2.6
Mn 0.4 2.3 2.3
Fe 3.6 4.9 4.9
Al 4.5 3.1 3.1
Crude lipid 42 4.4 7.5
Crude fibre 268 ≤ 0.3 3.2
Neutral detergent fibre 430 ≤ 0.3 4.7
Acid detergent fibre 362 ≤ 0.3 1.9
Acid detergent lignin 94 ≤ 0.3 4.9
DW = dry weight, CV = pooled coefficient of variation between replicate
analyses (n = 3 per heifer) and heifers (n = 5)
Freshly thawed faeces sub-samples (0.5 g DW) of heifer 2 were incubated for 28
days at 25°C in the dark in 40 replicates. For the determination of microbial biomass C
and N (final method, see below) and ergosterol four replicate faeces portions for each