Brazilian Oral Research - Sociedade Brasileira de Pesquisa ...

HA001
The effect of BMP-2 on the osteoconductive properties of
β-tricalcium phosphate in rat calvaria defects
Luvizuto ER*, Okamoto T, Sonoda CK, Queiroz TP, Souza FA, Pereira FP, Panzarini SR, Okamoto R
Cirurgia e Clínica Integrada - UNIVERSIDADE ESTADUAL PAULISTA - ARAÇATUBA.
E-mail: eloaluvizuto@hotmail.com
Bone formation in critical-sized calvaria defects is strongly dependent on the osteoconductive properties of
grafts. It remains a matter of controversy whether biomaterials can replace autografts and whether the supplementation
of biomaterials with Bone Morphogenetic Proteins (BMPs) is necessary to enhance bone formation.
We examined rat calvaria critical-sized defects (5mm diameter) treated with β-tricalcium phosphate (TCP;
Cerasorb ® M), polylactic and polyglycolic acid gel (PLA/PGA; Fisiograft ® ) and calcium phosphate cement
(CPC; Norian ® CRS ® ), either alone or in the presence of 5μg of BMP-2 after 45 days. Autografts and untreated
defects served as controls. Bone formation was evaluated based on μCT analysis, histomorphometric analysis
and fluorescence analysis. We report that TCP supported bone formation more efficiently than did autografts.
Bone formation in the presence of TCP alone reached a maximal level, as BMP-2 supplementation failed to
enhance bone formation. By contrast, no significant difference in bone formation was observed when PLA/
PGA and CPC were compared to autografts. Moreover, the presence of BMP-2 did not substantially change the
osteoconductive properties of PLA/PGA or CPC.
The osteoconductive properties of TCP are superior to those of autografts and that TCP does not require BMP-
2 supplementation. Our findings also show that the decreased osteoconductive properties of PLA/PGA and
CPC cannot be overcome by BMP-2 supplementation in rat calvaria defects. (Apoio: FAPESP - 2008/10516-6)
HA002 Long-term evaluation of secondary alveolar bone grafting after
canine eruption in complete unilateral cleft lip and palate
Calvo AM*, Carvalho RM, Bastos JUC, Lorenzoni DC, Ozawa TO
Ciências da Reabilitação - HOSPITAL DE REABILITAÇÃO DE ANOMALIAS CRANIOFACIAIS-
UNIVERSIDADE DE SÃO PAULO.
E-mail: dricalvo@usp.br
The aim of this study was to evaluate the result of secondary alveolar bone grafting (SABG) in patients with
complete unilateral cleft lip and palate (UCLP), operated on after the permanent canine eruption. Seventyfour
periapical radiographs from patients with complete UCLP (mean age 14 years) submitted to SABG, were
analyzed retrospectively for the amount of bone in the cleft site through the Bergland and Chelsea scales. The
average time of postoperative control was 1 year and 9 months. Of the cases, 47.3% was classified as Bergland
type I and Chelsea type A; 35.2% as type II/C; 6.7% as Type III/D, and 10.8% as Type IV/Failure. When the
canine was moved in the grafted area, the success index (Type I/A) was 56.8%; for the cases where it was kept
a space for implantation or prosthetic finishing, the index was 45.8%.
It was verified that even in advanced ages, after the eruption of the permanent canine, alveolar bone grafting
could be considered a high index of success procedure, especially when the graft is stimulated through orthodontic
movement.
HA005 Correspondence of Repetitive-element PCR and Multilocus
Sequence Typing for subtyping L. paracasei from human oral
biofilm
Parolo CCF*, Alves LS, Corção G, Maltz M
Odontologia Preventiva e Social - UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL.
E-mail: fatturiparolo@yahoo.com
Seventy five isolates of L.paracasei recovered from human dental biofilm formed in situ from 7 subjects
were typed by repetitive-element PCR (REP-PCR) and characterized by means of multilocus sequence typing
(MLST) by partial sequencing of seven housekeeping genes (fusA, ileS, lepA, leuS, pyrG, recA and recG). In this
study, a comparison of these subtyping methods was used to distinguish isolates from human source. There were
mixed results among REP-PCR patterns compared with the MLST. Different numbers of patterns were obtained
for L.paracasei according to the molecular technique used (14 MLST versus 25 REP-PCR patterns). For some
strains, REP-PCR was more discriminatory than MLST. In other cases, strains belonging to different sequence
types (ST) by MLST were grouped together by REP-PCR, showing that MLST was more discriminatory than
REP-PCR. In few cases MLST and REP-PCR presented the same discriminatory power. Differences in the
results between the typing methods, MLST and REP-PCR, are likely dependent on the way that genetic diversity
is measured. REP-PCR and MLST are considered reliable tools for identifying and discriminating clonal
groups among Lactobacillus strains. However, REP-PCR showed a greater discriminatory power in comparison
to MLST, but little agreement was observed between this two methods. Therefore, MLST could enhance the
genetic diversity obtained by REP-PCR.
The combination of MLST and REP-PCR increased the discriminatory power in detecting the genetic diversity
of L. paracasei. (Apoio: CNPq - 480841/2007-1)
HA006 Comparison between gross motor (GMFCS) and oral motor
(OMAS) functions in children with cerebral palsy
Ortega AOL*, Ciamponi AL, Santos MTBR
Estomatologia - UNIVERSIDADE DE SÃO PAULO - SÃO PAULO.
E-mail: aliraort@uol.com.br
Cerebral palsy (CP) is the result of a non-progressive damage which occurs during brain development. The
method of assessing clinical types of CP based on the performance of gross motor activities has been the most
commonly applied. This assessment scale, known as GMFCS (Gross Motor Function Classification System),
classifies functional characteristics into five levels. In order to assess oral motricity, a validated scale known
as Oral Motor Assessment Scale (OMAS) was used. This paper aimed to establish a correlation between the
general and oral motor skill in children with CP. Both scales were used in fifty three subjects aged 3 to 13
(8.5±3.2), of both genders. When GMFCS and OMAS scores were compared, a significant association was
observed between functionality (OMAS 2 and 3) and GMFCS I and II and non-functionality (OMAS 0 and
1) and GMFCS III, IV, and V (p