2008 Savremena poljoprivreda 57 1-2.indd - Poljoprivredni fakultet ...
2008 Savremena poljoprivreda 57 1-2.indd - Poljoprivredni fakultet ...
2008 Savremena poljoprivreda 57 1-2.indd - Poljoprivredni fakultet ...
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CHRENEK P., VAŠÍČEK D., MAKAREVICH A., GAJARSKÁ T., GASTNEROVÁ I., BULLA<br />
J.: Detection of human protein C gene integration transgenic rabbits by polymerase chain reaction.<br />
Vet. Med.– Czech 44: (3), 79–82, 1999.<br />
CHRENEK P., VAŠÍČEK D., MAKAREVICH A., UHRÍN P., PETROVIČOVÁ I., LUBON H.,<br />
BINDER BR., BULLA J.: Integration and expression of the WAP-hPC gene in three generations of<br />
transgenic rabbits. Czech J. Anim. Sci. 47: (2), 45–49, 2002.<br />
CHRENEK P., VAŠÍČEK D., MAKAREVICH A., JURČÍK R., SUVEGOVÁ K., BAUER M.,<br />
RAFAY J., BULLA J., HETÉNYI L., ERICKSON J., PALEYANDA R. K.: Stability of transgene<br />
transmission in three generations of transgenic rabbits after single or double pronuclear microinjection.<br />
8th WRC, Mexico, September 44–50, 2004.<br />
DRAGIN S., CHRENEK P., BOZIC, A.: Effects of transgenesis on F2 and F3 rabbit offspring generation<br />
development. 5. Sci. conference of PhD. students, FPV – UKF Nitra, Slovakia, Marec 28–<br />
32, 2004.<br />
DUBY R.T., CUNNIFF M.B., BELAK J.M., BALIS J.J., ROBOL J.M.: Effect of milking frequency<br />
on collection of milk from nursing New Zealand white rabbits. Anim. Biotechnol. 4: 31–42,<br />
1993.<br />
HODGSON, J.: Whole Animals for Wholesale Protein Production. Bio/Technology 10: 863–866,<br />
1992.<br />
SCHRANNER S.: Untersuchungen zum maschinellen Milchentzug beim Kaninchen als Grandlage<br />
zur Bestimmung von Laktationsleistungen und Milchinhaltsstoffen. Inaugural-Dissertation,<br />
Ludwig-Maximilians-Universität München, 120, 1993.<br />
VAN COTT E.K., LUBON H., GWAZDAUSKAS F.C., KNIGHT J., DROHAN W.N., VELAN-<br />
DER W. H.: Recombinant human protein C expression in milk of transgenic pigs and effect on endogenious<br />
milk immunoglobulin and transferin levels. Transgenic Res. 10: 43–51, 2001.<br />
PROIZVODNJA HUMANOG PROTEINA C<br />
U MLEKU F3 GENERACIJE TRANSGENIH KUNIĆA<br />
PETER CHRENEK, SAŠA DRAGIN, ALEKSANDAR BOŽIĆ<br />
Izvod<br />
U radu je ispitana stabilnost transmisije transgena, proizvodnja mleka i sadržaj<br />
rekombinantnog humanog proteina C (rhPC) kod transgenih kunića F3 generacije. Transgeni<br />
kunići su nosili 4,2 kb mišjeg proteina surutke kao promotera i 9,4 kb genske knstrukcije<br />
humanog proteina C. Životinje su dobijene ukrštanjem F2 generacije transgenih i<br />
netransgenih kunića. PCR analiza uzoraka mleka F3 generacije je pokazala prisustvo<br />
transgena. Proizvodnja mleka dobijenog metodom merenje-sisanje-merenje nije pokazala<br />
značajne razlike između transgenih i netransgenih jedinki. Detekcija metodom Western<br />
blott i ELISA test su potvrdili prisustvo rhPC kod transgenih životinja, dok su uzorci netransgenih<br />
kunića bili negativni. U zaključku se potvrđuje stabilnost transgene transmisije<br />
hPC i produkcija rhPC u F3 generaciji transgenih kunića sa istom efi kasnošću kao i u<br />
F1 i F2 generaciji bez smanjenja proizvodnje mleka.<br />
Ključne reči: rhPC, transgeneza, kunići, proizvodnja mleka<br />
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