Volume III, Appendices EM - National Marine Fisheries Service ...

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Appendix 7. Petroleum Hydrocarbon Tissue Sampling Protocol Supplies for sampling All instruments used in handling (e.g. scalpels and forceps, cutting boards) or storing (e.g. jars, foil, sheets) samples must be made of a non-contaminating material consisting of stainless steel, glass, Teflon, or aluminum. - Solvent-rinsed glass containers with Teflon-lined lids for tissues - Solvent-rinsed Teflon sheets for tissues - Aluminum foil (if Teflon sheets are not available) sample to the dull side - Sterile syringes and needles - Amber glass vials or glass vials covered with foil with Teflon lids (for bile, urine) - Teflon screw top vials (for blood storage and urine) - Stainless steel scalpels, knifes, forceps - Isopropyl alcohol (99.9% pesticide free IPA) to rinse instrument - Wooden tongue depressors (can be used to handle tissues if necessary) - Whirl-pak bags or Zip-lock freezer bags - 10% buffered formalin and appropriate containers for histopathology samples - Permanent marker or pen - Evidence/Custody tape and labels - Sample Log/Chain of Custody forms Sampling Protocol Tissues to collect for petroleum hydrocarbon analysis in order of preference: a. bile b. urine c. whole blood d. stomach and intestinal contents e. blubber/fat f. liver g. kidney h. lung i. intestine j. brain k. muscle i. Samples taken for analysis should only be collected from alive or freshly dead animals. If a necropsy cannot be performed within 24 hrs after death, the carcass should be frozen for later examination. ii. Recommended minimum sample size is 10-20 g of tissues (approx. 1-2 tablespoons) and 5 ml for fluids (blood, urine, bile, feces, stomach contents). However, analysis can be performed on as little as 100 L of bile; therefore collect whatever amount is present.
Appendix 7. Petroleum Hydrocarbon Tissue Sampling Protocol, page 2 iii. Fluids such as blood, urine, and bile should be collected using sterile syringes or pipettes and transferred to Teflon vials (blood) or amber glass vials (bile, urine). iv. Use powder-free nitrile gloves. Vinyl gloves are an acceptable alternative. Avoid contact of gloves with samples. v. Scalpels, knifes, and cutting tools used for tissue collection should be cleaned and rinsed with isopropyl alcohol between tissues. If heavily contaminated with oil, instruments can be cleaned with detergent (e.g. Dawn), rinsed with water, and then rinsed with alcohol. vi. Samples are stored preferably in solvent-rinsed Teflon-lined glass jars, labeled, and secured with evidence tape/custody seal. If glass jars are not available, samples can be placed in Teflon sheets or aluminum foil (dull side to sample) and stored in whirlpaks/freezer bags. vii. If samples/tissues have come in contact with a contaminating material (e.g. plastic bag), collect and store a representative example of that material (e.g. plastic bag) using the same method as for collecting tissues. viii. Collect a representative sample of each tissue (< 1 cm thick) preserved in 10% buffered formalin for histopathology. Duplicate hydrocarbon and histology samples whenever possible. ix. Each sample must be labeled with Spill Name, Log #, Level A Field #, Species, Tag#, Arrival Date, Sampling Date, and Capture Location and securely stored. x. Samples for PAH analysis should be chilled immediately on ice/refrigeration and then frozen as soon as possible to -20ºC or colder in a locked freezer. Histopathology samples are stored at room temperature. All evidence should be securely stored and refrigerated/frozen until the Wildlife Branch Director provides further instructions. If samples are transferred to a different location or sent for analysis, a Chain of Custody form is required. A Chain of Custody form can be found in this document, but are often provided by the laboratory. Shipping: Ship samples frozen on blue ice or with ~5 lbs dry ice according to laboratory specification using Federal Express (FedEx). FedEx follows IATA regulations for shipping hazardous materials and maintains chain of custody record by tracking packages. Sampling supplies such as jars, label, and custody seals are often supplied by the analytical laboratory and are produced by: I-Chem Brand, Certified 300 Series jars Order: 1-800-451-4351, www.ichembrand.com
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Draft Programmatic Environmental Im
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Table E-1. Protected and Sensitive
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Bird Island Marine Sanctuary Table
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Table E-7. Protected Fisheries Reso
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Common Name Table E-10. Protected B
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Common Name Table E-12. Protected B
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Common Name Guam bridled white-eye
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Table E-14. Marine Mammals Common i
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Marine Mammal Stranding Network Org
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Organization/Individual NMFS Southw
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Organization/Individual NMFS Pacifi
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Individual NMFS Southeast Region (c
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Individual NMFS Alaska Region Organ
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Individual NMFS Pacific Islands Reg
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Teri Rowles, D.V.M., Ph.D. National
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Authorization UNITED STATES DEPARTM
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PROJECT I1 - ENHANCEMENT ACTIVITIES
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. Euthanasia 1) 2) The NMFS Nationa
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g. The Holder/PI must not harass or
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meets the minimum standards of coll
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2) Live captures; Stranding respons
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4. Persons who require state, Feder
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1 1. Any falsification of informati
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Florida Keys National Marine Sanctu
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Seattle, Washington 981 58 Blaine 9
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50 CFR $21 6.37 Marine mammal parts
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APPENDIX H GENERAL DESCRIPTIONS OF
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oats. Animals are located by having
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In some cases, animals may need to
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ecorders (TDRs), life history trans
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LHX tags are satellite linked, dela
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analyzed. During live animal respon
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1.1.12 Ultrasound Sampling Ultrasou
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interfaced to a laptop computer. Th
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Recording, ground, and reference su
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2. Future ESA/MMPA Permit Activitie
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3. References Dierauf 1994 Dierauf,
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4. Acronyms ABR Auditory Brainstem
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Table 1. Proposed activities over a
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Table 1. Proposed activities over a
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PERSISTENT CONTAMINANTS IN SELECTED
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Table 2. Metadata for Persistent Or
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(PCDDs) oxygen atoms. Like PCBs, PC
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Although no physiologic requirement
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II.B. Concentrations of environment
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species targeted in this review, Ca
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data exist pertaining to contaminan
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_____, 2002c. Draft toxicological p
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Houde, M, Wells, R, Fair, P, Bossar
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Neale, J., Gulland, F., Schmeltzer,
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Tuerk, K, Kucklick, J., Becker, P.,
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Table 1. Summary of Concentrations
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Table 3. Polychlorinated dibenzo-p-
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Appendix III. Mercury, Cadmium, Lea
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Cadmium (Cd) (continued) Species Ti
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Lead (Pb) (continued) Mean Date Spe
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h Standard error of the mean i For
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Euthanasia Questionnaire Response S
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Prescott Overview FY01-07 Running T
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YEAR STATE APPLICANT TITLE 2002 200
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YEAR STATE APPLICANT TITLE FEDERAL
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YEAR STATE APPLICANT TITLE 2005 FL
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YEAR STATE APPLICANT TITLE FEDERAL
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APPENDIX L MARINE MAMMAL OIL SPILL
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Page 166 and 167: NOTICE MARINE MAMMAL OIL SPILL RESP
Page 168 and 169: MARINE MAMMAL OIL SPILL RESPONSE GU
Page 172 and 173: Organizational Structure Organizati
Page 176 and 177: Stranding Network and Facility Requ
Page 180 and 181: Sample Collection and Label: It is
Page 184 and 185: Wildlife Recovery and Transportatio
Page 188 and 189: Intake Procedures Initial Intake Pr
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Page 196 and 197: Disposition Release The goal in reh
Page 206 and 207: Appendix 1. Search Effort Log Searc
Page 208 and 209: Appendix 2b. Live Marine Mammal Dat
Page 210 and 211: Appendix 3b. Dead Marine Mammal Dat
Page 212 and 213: Appendix 5. Oiled Marine Mammal Dai
Page 216 and 217: Appendix 8. Oil Spill Response Labo
Page 218 and 219: Appendix 10. Chain of Custody Form
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Page 223 and 224: Table 1: Summary of overall statewi
Page 225 and 226: Table 3: Summary of statewide infor
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