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3.4.5 Classification of the survey data<br />

CHaPTeR 3 Method of data analysis and collection<br />

Classification analyses for this study were primarily carried out within the DOS version of<br />

the PATN software package (Belbin 1994). Additional analyses, such as fidelity analysis<br />

(Keith & Bedward 1999), were carried out using queries in Microsoft Office Access (Microsoft<br />

Corporation 2003). How the analysis dataset that was used in the classification process was<br />

derived is described above in section 3.4.4.<br />

For all analyses other than the preliminary exploratory analyses, the Bray-Curtis measure of<br />

association was used due to its appropriateness when analysing cover / abundance scores (see<br />

Section 3.3.1 above).<br />

An initial agglomerative hierarchical classification of the data into groups was carried out<br />

using the FUSE module in PATN (Belbin 2005). A Flexible UPGMA strategy was applied to this<br />

classification with a beta left at the software default of -0.1. This approach was used to produce<br />

150 and 300 groups as it was felt that the final number of communities would be between these<br />

values; this allowed for an understanding of the broader patterns and relationships between<br />

the finer groups to assist in the merging of these groups.<br />

Initial analyses of the outputs of this first iteration of the classification suggested further issues<br />

with the dataset which had not previously been detected. The dataset was further refined,<br />

with some sites being removed due to suspected biases or errors, and cover scores for some<br />

data being re-standardised. In addition to these refinements, data from all groups which had<br />

been defined for sites with less than 10 species were removed before the second iteration to<br />

remove any possible effects these sites may have had on the overall classification. These groups<br />

were reintroduced to the classification once the remaining data had been interpreted. A second<br />

iteration of the hierarchical classification of 150 and 300 groups was then carried out using the<br />

FUSE module in PATN.<br />

In parallel to this approach, a non-hierarchical classification was also carried out using the ALOC<br />

module in PATN (Belbin 1995) in order to compare the outputs from these two approaches. Nonhierarchical<br />

classification has been suggested as more appropriate for use in cases where a very<br />

large dataset is to be analysed (Belbin 1995); however, in this case the groups produced were<br />

less intuitive and the outputs of this approach were only used to compare with the hierarchical<br />

classification and assist in identifying possible misclassifications.<br />

3.4.6 Interpretation and refinement of classification<br />

The outputs of the analyses outlined above were then interpreted and refined to produce a<br />

draft classification scheme to present to the expert review panel. To support the interpretation<br />

of the results dendrograms were produced at both the 300 and 150 group levels, allowing an<br />

assessment of relationships between groups (see Appendix 4). Species fidelity statistics were<br />

also produced following the approach described by Keith and Bedward (1999) to assist in<br />

interpreting and defining groups and identifying possible misclassified sites.<br />

Various environmental data were also analysed to support the interpretation of the classification<br />

outputs. Site locations were loaded into a GIS to allow the spatial distribution of sites within each<br />

group to be assessed and possible geographic outliers to be identified. A range of environmental<br />

parameters was also captured in a GIS for each site, allowing this information to be summarised<br />

for each proposed group. Parameters captured included topography (altitude, slope and<br />

aspect), climate (mean annual temperature and rainfall), geology and lithology. In addition to<br />

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