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3.4.5 Classification of the survey data<br />
CHaPTeR 3 Method of data analysis and collection<br />
Classification analyses for this study were primarily carried out within the DOS version of<br />
the PATN software package (Belbin 1994). Additional analyses, such as fidelity analysis<br />
(Keith & Bedward 1999), were carried out using queries in Microsoft Office Access (Microsoft<br />
Corporation 2003). How the analysis dataset that was used in the classification process was<br />
derived is described above in section 3.4.4.<br />
For all analyses other than the preliminary exploratory analyses, the Bray-Curtis measure of<br />
association was used due to its appropriateness when analysing cover / abundance scores (see<br />
Section 3.3.1 above).<br />
An initial agglomerative hierarchical classification of the data into groups was carried out<br />
using the FUSE module in PATN (Belbin 2005). A Flexible UPGMA strategy was applied to this<br />
classification with a beta left at the software default of -0.1. This approach was used to produce<br />
150 and 300 groups as it was felt that the final number of communities would be between these<br />
values; this allowed for an understanding of the broader patterns and relationships between<br />
the finer groups to assist in the merging of these groups.<br />
Initial analyses of the outputs of this first iteration of the classification suggested further issues<br />
with the dataset which had not previously been detected. The dataset was further refined,<br />
with some sites being removed due to suspected biases or errors, and cover scores for some<br />
data being re-standardised. In addition to these refinements, data from all groups which had<br />
been defined for sites with less than 10 species were removed before the second iteration to<br />
remove any possible effects these sites may have had on the overall classification. These groups<br />
were reintroduced to the classification once the remaining data had been interpreted. A second<br />
iteration of the hierarchical classification of 150 and 300 groups was then carried out using the<br />
FUSE module in PATN.<br />
In parallel to this approach, a non-hierarchical classification was also carried out using the ALOC<br />
module in PATN (Belbin 1995) in order to compare the outputs from these two approaches. Nonhierarchical<br />
classification has been suggested as more appropriate for use in cases where a very<br />
large dataset is to be analysed (Belbin 1995); however, in this case the groups produced were<br />
less intuitive and the outputs of this approach were only used to compare with the hierarchical<br />
classification and assist in identifying possible misclassifications.<br />
3.4.6 Interpretation and refinement of classification<br />
The outputs of the analyses outlined above were then interpreted and refined to produce a<br />
draft classification scheme to present to the expert review panel. To support the interpretation<br />
of the results dendrograms were produced at both the 300 and 150 group levels, allowing an<br />
assessment of relationships between groups (see Appendix 4). Species fidelity statistics were<br />
also produced following the approach described by Keith and Bedward (1999) to assist in<br />
interpreting and defining groups and identifying possible misclassified sites.<br />
Various environmental data were also analysed to support the interpretation of the classification<br />
outputs. Site locations were loaded into a GIS to allow the spatial distribution of sites within each<br />
group to be assessed and possible geographic outliers to be identified. A range of environmental<br />
parameters was also captured in a GIS for each site, allowing this information to be summarised<br />
for each proposed group. Parameters captured included topography (altitude, slope and<br />
aspect), climate (mean annual temperature and rainfall), geology and lithology. In addition to<br />
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