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THE FLORIDA STATE UNIVERSITY ARTS AND SCIENCES ...

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Using this method the percent of loss of nitrogen due to denitrification is obtained for the<br />

study areas in Jacksonville (Fl). While it was difficult to know the initial nitrate<br />

concentrations we could assume the standard of 35mg/l/d or the highest nitrate<br />

concentration value to be C0. As this is a very conservative estimate the result estimated<br />

denitrification rate would be a minimum possible loss. Based on the above methodology<br />

and assumptions we estimated 26 - 82% of nitrate removed in the Jacksonville area that<br />

can be attributed to denitrification.<br />

6.3. Use of isotopes to estimate the loss of Nitrogen due to<br />

denitrification.<br />

Between September 2009 and December 2010 samples were collected from three study<br />

locations and an isotopic analysis is conducted at the Colorado Plateau Analytical<br />

Laboratory, Arizona. The results are applied to the study sites<br />

The data shows that denitrification is taking place in all the study areas, all of the regions<br />

show an increase in δ 18 O as δ 15 N increases.<br />

δδ 18 O<br />

30.00<br />

25.00<br />

20.00<br />

15.00<br />

10.00<br />

5.00<br />

y = 0.4394x + 1.8219<br />

R 2 = 0.9105<br />

δ 18 O Vs. δ 15 N<br />

0.00<br />

0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00<br />

δ 15 N<br />

Figure 6.2 δ 18 O vs. δ 15 N Eggleston Heights (April 2010).<br />

159

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