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THE FLORIDA STATE UNIVERSITY ARTS AND SCIENCES ...

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isotopes can be particularly useful in estimating the percent of nitrogen loss due to<br />

denitrification. Research into the use of stable isotopes will need to be conducted to be<br />

able to determine denitrification rates using stable isotopes. Research into the use of<br />

stable isotopes may perhaps lead to an alternative method to obtain field denitrification<br />

rates and thus allowing for the current gap in field denitrification rated to be filled.<br />

In précis one may say that, the inability to develop a complete model to predict<br />

denitrification on a field scale is not an issue of model development as much as it is a<br />

matter of developing a good and robust technique to measure denitrification rates on both<br />

the lab and field scale. The methodology shown in this work clearly demonstrates that<br />

the basic principles on denitrification are well understood and that there are simple<br />

methods that can deal with such a complex problem. The development of an accurate<br />

field scale model thus depends on precise data being used and to obtain this precise data<br />

especially on the lower end of the scale a refined technique is required.<br />

Perhaps Allison (1955) is correct when he observed that in spite of several years of<br />

research while the main mechanisms of nitrogen loss are known, the quantitative type of<br />

data related to each type of loss are still inadequate. Davidson and Seitzinger (2006)<br />

certainly agree that the observations are still valid five decades later. One of the possible<br />

reasons for this apparent lack of progress is that the process of denitrification requires<br />

integration across several disciplines and scales. It can be said with some certainty that;<br />

there is no single methodological procedure that will solve the enigma of balancing<br />

nitrogen budgets. However there could be a universal approach to quantifying<br />

denitrification, ANNs are one such promising approach to quantifying denitrification<br />

(Oehler, 2010).<br />

172

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