Report No xxxx - Instytut Fizyki JÄdrowej PAN

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DESICCATION RESISTANCE OF THE LICHEN TURGIDOSCULUM COMPLICATULUM AND ITS PHOTOBIONT PRASIOLA CRISPA BY PROTON MAGNETIC RELAXATION, AND SORPTION ISOTHERM * H. Harańczyk, *A. Ligęzowska, **M. Olech * Institute of Physics and **Institute of Botany, Jagiellonian University, Kraków, Poland The Antarctic lichens, as the organisms experiencing the extreme low temperatures and low hydration level in their habitat, are proper system in the investigations of life extremes for deep desiccation stress. In contrast to vascular plants they may perform active photosynthetic process even if their thallus is frozen. To emphasize the range of their adaptation, it is worth mentioning that some of lichen species survive the freezing down to the temperature of liquid nitrogen intependently on the freezing rate; they perform the active photosynthesis below 0 0 C and even below the temperature of the heterogeneous ice nucleation in their cellular fluids [Kappen, 1993, Kieft and Ahmadijan, 1989, Kieft and Ruscetti, 1990]; they stimulate ice deposition in extracellular spaces of thallus [Schroeter and Scheidegger, 1995, Harañczyk et al., 2003a]; and prevent the intracellular spaces from ice nucleation by transfer of the freezing loosely bound water to the not freezing tightly bound water pool [Harańczyk et al., 2000, 2003b]; they reversibly dehydrate down the two dimensional percolation threshold of water bound on the thallus surfaces [Harañczyk, 2003]; they can hydrate from the gaseous phase up to the level sufficiently high to induce the photosynthetic activity; the take water directly from the snow up [Kappen et al, 1991]. Essential for understanding the molecular mechanism of the metabolic recovery during rehydration is knowledge about the number and distribution of water binding sites, sequence and kinetics of their saturation, as well as fromation of tightly and loosely bound water pools at different steps of hydration process. In our experiments we investigated the lichen Turgidosculum complicatulum and its free living fotobiont an alga Prasiola crispa. The relative independence of symbionts [Kovaeik & Pereira, 2001] makes an unique possibility to test the dessication resistance of both partners separately. Samples were collected in Maritime Antarctic, Antarctic Peninsula, King George Island, Polish Antarctic H. Arctowski Station. We measured the initial stages of mild rehydration from the gaseous phase of the lichen thallus o T. complicatulum and the separately living (in the same habitat) alga P. crispa using hydration kinetics, adsorption isotherm and high power proton free induction decays (FIDs). Four different water pools are differentiated: (i) water bound to the ‘primary’ water binding sites (∆m/m 0 = 0.020±0.005 and ∆m/m 0 = 0.030±0.002, for P. crispa and for T. complicatulum, respectively) which was not removed by incubation over silica gel; (ii) and (iii) two fractions of tightly bound water, differentiated by hydration constant (∆m/m 0 = 0.007±0.001 and ∆m/m 0 = 0.10±0.02 for P. crispa; and ∆m/m 0 = 0.07±0.02 and the one exceeding ∆m/m 0 = 0.10 for T. complicatulum); and (iv) loosely bound water pool. Both fractions of tightly bound water fractions are not differentiated by molecular mobility but only by the binding strength. 32
References: 1. H. Harańczyk On water in extremely dry biological systems, Wyd. Uniwersytetu Jagiellońskiego (2003). 2. H.Harańczyk, S.Gaździński, M.Olech, In: New Aspects in Cryptogamic Research, Contribution in Honour of Ludger Kappen. Bibl. Lichenol., 75, 265 (2000). 3. H.Harańczyk, J.Grandjean, M.Olech Colloids & Surfaces, B: Biointerfaces 28/4, 239 (2003a). 4. H.Harańczyk, J.Grandjean, M.Olech, M.Michalik Colloids & Surfaces, B: Biointerfaces 28/4, 251 (2003b). 5. L. Kappen, Arctic, 46, 297 (1993). 6. L. Kappen, M. Breuer, M. Bölter, Polar Biology, 11, 393 (1991). 7. T.L. Kieft, V. Ahmadjian, Lichenologist, 21, 355 (1989). 8. T.L. Kieft, T. Ruscetti, J. Bacteriol., 172, 3519 (1990). 9. L. Kovaeik, A.B. Pereira, <strong>No</strong>va Hedwigia, Beiheft 123, 465 (2001). 10. B. Schroeter, Ch. Scheidegger, New Phytol., 131, 273 (1995). 33
Page 1 and 2: The Henryk Niewodniczański INSTITU
Page 3 and 4: Contents R. Banyś, A. Słowik, M.
Page 5 and 6: J. Kolmas, M. Uniczko, E. Kalinowsk
Page 7 and 8: Ł. Popenda, Z. Gdaniec, G. Dominia
Page 9 and 10: USEFULNESS OF PROTON MAGNETIC RESON
Page 11 and 12: INFLUENCE OF PARAMAGNETIC IONS ON F
Page 13 and 14: NMR RELAXATION OF PAPER SAMPLES Bar
Page 15 and 16: SPIN-LATTICE RELAXATION OF D 2 QUAN
Page 17 and 18: INVESTIGATION OF NEUROPROTECTING EF
Page 19 and 20: OPTICAL PUMPING OF 3 He Katarzyna C
Page 21 and 22: NMR STUDY OF GELATION PROCESS OF LO
Page 23 and 24: MRI INVESTIGATIONS OF HYDROGEL FORM
Page 25 and 26: CHEMICAL SHIFT TITRATION AT THE INT
Page 27 and 28: LOCAL DYNAMICS AND ORGANIZATION OF
Page 29 and 30: 1 H NMR DETECTION OF σ-ADDUCTS IN
Page 31: HYDRATION OF WHEAT PHOTOSYNTHETIC M
Page 35 and 36: (EDV) and end-systolic (ESV) volume
Page 37 and 38: THE MOST STABLE POLYMORPHIC FORM OF
Page 39 and 40: A DETERMINATION OF ABSOLUTE SHIELDI
Page 41 and 42: DIAGNOTIC VALUE OF MRI IN CONVERSIO
Page 43 and 44: NMR AND FTIR STUDY OF MOLECULAR DYN
Page 45 and 46: EFFECTS OF INTERMOLECULAR INTERACTI
Page 47 and 48: 600000 Integral of 1D MRI profils [
Page 49 and 50: simultaneous analyse of the tempera
Page 51 and 52: 1 H NMR STUDIES OF PLATINUM(II) CHL
Page 53 and 54: 35 Cl-NQR STUDY OF ELECTRONIC STRUC
Page 55 and 56: 35 Cl- NQR AND 1 H-NMR STUDY OF MOL
Page 57 and 58: THE SYNTHESIS AND NMR ANALYSIS OF T
Page 59 and 60: STUDY OF MOTIONAL PROCESSES OF DRAW
Page 61 and 62: intensity (arb. units) 0,20 0,15 0,
Page 63 and 64: data for Cβ-nonplanar model 3 are
Page 65 and 66: T 1 DISPERSION AND SELF-DIFFUSION N
Page 67 and 68: A LOW FIELD MRI SYSTEM FOR HYPERPOL
Page 69 and 70: 1 H MAS NMR OF FLAVONOIDS Katarzyna
Page 71 and 72: EFFECT OF TEMPERATURE ON LIPOSOME S
Page 73 and 74: 1 H, 13 C NMR AND GIAO/DFT CALCULAT
Page 75 and 76: NMR STUDY OF THE HUMIC ACIDS EXTRAC
Page 77 and 78: NMR STUDY OF LAYERED ANGANITE La 1.
Page 79 and 80: MAGNETIC SUSCEPTIBILITY EVALUATION
Page 81 and 82: A NOVEL SOURCE OF MAGNETIC FIELD FO
Page 83 and 84:
DETERMINATION OF AMINO ACIDS IN BOD
Page 85 and 86:
MOLECULAR DYNAMICS OF TERT-BUTYL CH
Page 87 and 88:
MR MICROSCOPY IN COMPARISON OF YOUN
Page 89 and 90:
NH CH CH 2 NH CH CO COONa n CH 2 m
Page 91 and 92:
35 Cl-NQR STUDY OF MOLECULAR DYNAMI
Page 93 and 94:
35 Cl-NQR STUDY OF THE SUBSTITUENT
Page 95 and 96:
13 C AND 1 H NUCLEAR MAGNETIC SHIEL
Page 97 and 98:
13 C CPMAS NMR OF ANTHOCYANINS AND
Page 99 and 100:
INVESTIGATION OF TRANSESTERIFICATIO
Page 101 and 102:
T 2 RELAXATION MAP OF ARTICULAR CAR
Page 103 and 104:
MICROTOMOGRAPHY STUDIES OF TABLETS
Page 105 and 106:
MAPPING OF THE BI-EXPONENTIAL DIFFU
Page 107 and 108:
DEUTERIUM NMR IN RMn 2 D 2 (R = Y,
Page 109 and 110:
The neutron scattering (IINS, QNS,
Page 111 and 112:
STRUCTURE OF 4-QUINOLINONES ANALYSE
Page 113:
NMR MULTIPOLE RELAXATION IN THE ROT
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Report No xxxx - Instytut Fizyki JÄdrowej PAN