Die Embryonalentwicklung der Paradiesschnecke ... - TOBIAS-lib

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Kapitel 1 Fig. 3: Pt effect in Ca-supplemented Marisa cornuarietis and in Planorbarius corneus. (a) M. cornuarietis with a small, external shell, 11 days post fertilization, exposed to 200 µg/L PtCl 2 and equimolar concentrations of Ca 2+ , ma: mantle, gi: gills. (b) P. corneus without external shell, 23 days post fertilization, exposed to 300 µg/L PtCl 2. Scale bars are 500 µm. 26.4 µg/g Pt. In contrast to Pt 2+ , the physicochemical similar ion Pd 2+ did not affect shell formation during embryogenesis. Only occasionally were we able to induce shell-less M. cornuarietis embryos with high concentrations of Li + : 2.5 mg/L LiCl caused 10.0 ± 6.0% shell-less snails and 3 mg/L LiCl caused 20.0 ± 9.5% shell-less snails. The fate of the shell secreting edge of the mantle fold and the formation of an internal shell Since we found DAB to specifically stain the shell-secreting peripheral edge of the mantle fold, we were able to follow the fate of the tissue, which usually forms the mantle fold edge during embryogenesis and to visualize the presumably shell-secreting region of Pt-exposed M. cornuarietis. In contrast to the controls, the tissue archetypically designated to form the mantle edge did neither evaginate nor overgrow the visceral sac but remained at the posterior end of the embryo and invaginated into the body (Fig. 4, c–f), thus closing the aperture of the shell gland. Using SEM, the morphology of the shell gland and the initial protoconch of continuously Pt-exposed embryos and water controls did not differ sub- 44
Kapitel 1 Fig. 4 4 : Location of the shell-secreting peripheral edge of the mantle fold in Marisa cornuarietis exposed to 200 µg/L PtCl2 and in controls. Scanning electron micrographs of Pt-exposed (200 µg/L PtCl2) M. cornuarietis and controls. (a) Control, 3 days post fertilization. (b) Pt-exposed MM. cornuarietis, 4 days post fertilization. (c) Control, 5 days post fertilization. (d) Pt-exposed M. cornuarietis, 6 days post fertilization. DAB staining (arrows) of the shell-secreting mantle edge of a control individual (e) and a Pt-exposed individual (f), both 5 days post fertilization. Scale bars are 100 µm. stantially (Fig. 4, a and b). However, while the shell grows rapidly during the subsequent days of embryogenesis in controls, the opening of the shell gland of Pt-exposed individuals was reduced in size. To test whether this effect and the subsequent lack of an external shell was possibly based on a lack or a drastic diminution of biomineralization activity during the days 4 <strong>Die</strong> Abbildungen 4e und f stammen von Raphaela Osterauer, die Bildtafel wurde von Raphaela Osterauer erstellt. 45
Page 1 and 2: Die Embryonalentwicklung der Paradi
Page 3 and 4: Inhaltsverzeichnis Zusammenfassung
Page 5 and 6: Zusammenfassung Hintergrund und Zie
Page 7 and 8: Hintergrund und Zielsetzung der Arb
Page 9 and 10: Hintergrund und Zielsetzung der Arb
Page 11 and 12: Material und Methoden Marisa cornua
Page 13 and 14: Material und Methoden Hälterung Di
Page 15 and 16: Material und Methoden lindividuen a
Page 17 and 18: Ergebnisse lung von Marisa cornuari
Page 19 and 20: Ergebnisse Kapitel 3: Marschner, L.
Page 21 and 22: Ergebnisse Wachstum des Columellamu
Page 23 and 24: Diskussion Naticidae) entwickelt si
Page 25 and 26: Diskussion engrailed und dpp-BMP2/4
Page 27 and 28: Diskussion Abb. 4: Vergleich der mi
Page 29 and 30: Schlussfolgerung perimentell zu unt
Page 31 and 32: Literatur Engelhardt, W. (2008). Wa
Page 33 and 34: Literatur Ponder, W. F. und Lindber
Page 35 and 36: Kapitel 1:Turning snails into slugs
Page 37 and 38: Kapitel 1 dae). Their ontogeny also
Page 39 and 40: Kapitel 1 Exposure experiments Test
Page 41 and 42: Kapitel 1 et al. (2001, 2003) or wi
Page 43 and 44: Kapitel 1 for 4 × 5 min with PTw (
Page 45 and 46: Kapitel 1 treatment 29.9 ± 32.78%
Page 47: Kapitel 1 Fig. 2 3 : Images of M. c
Page 51 and 52: Kapitel 1 internal solid circular s
Page 53 and 54: Kapitel 1 ietis as it was shown for
Page 55 and 56: Kapitel 1 Burnett, L.E., Woodson, P
Page 57 and 58: Kapitel 1 Page, L.R., 2002. Ontogen
Page 59 and 60: Kapitel 2: Arresting mantle formati
Page 61 and 62: Kapitel 2 tropods (Crofts, 1937, su
Page 63 and 64: Kapitel 2 Scanning electron microsc
Page 65 and 66: Kapitel 2 called “mantle anlage
Page 67 and 68: Kapitel 2 Fig. 2: M. cornuarietis,
Page 69 and 70: Kapitel 2 and the visceral sac rema
Page 71 and 72: Kapitel 2 Seven-day-old embryos At
Page 73 and 74: Kapitel 2 Fig. 7: M. cornuarietis,
Page 75 and 76: Kapitel 2 gation of internal shells
Page 77 and 78: Kapitel 2 first tended to invaginat
Page 79 and 80: Kapitel 2 side of the visceral sac
Page 81 and 82: Kapitel 2 cornuarietis (Mesogastrop
Page 83 and 84: Kapitel 3: External and internal sh
Page 85 and 86: Kapitel 3 during embryonic developm
Page 87 and 88: Kapitel 3 Differential growth of ma
Page 89 and 90: Kapitel 3 sac, facing away from the
Page 91 and 92: Kapitel 3 Fig. 4 9 : SEM-images of
Page 93 and 94: Kapitel 3 Fig. 6: Histological sect
Page 95 and 96: Kapitel 3 side of the visceral sac
Page 97 and 98: Kapitel 3 different mechanism that
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Kapitel 3 whether the inhibition of
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Kapitel 3 tinuum of gradual morphol
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Kapitel 3 served one. However, ther
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Kapitel 3 hand and modified in Inks
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Kapitel 3 ticipated in designing th
Page 109 and 110:
Kapitel 3 Demian, E. S. and Yousif,
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Kapitel 3 Page, L. R. (2003). Gastr
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Kapitel 4 Herewith, we were able to
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Kapitel 4 Histology and 3D reconstr
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Kapitel 4 72 or 96 hours at 6 ◦ C
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Kapitel 4 tioned on the ventral sid
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Kapitel 4 P2 does not curve backwar
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Kapitel 4 which this nerve is attac
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Kapitel 4 Fig. 5 13 : 3D reconstruc
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Kapitel 4 system in which the right
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Kapitel 4 ready present during tors
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Kapitel 4 Haydon, P. G., McCobb, D.
Page 133 and 134:
Kapitel 5: Quantifikation der Hsp70
Page 135 and 136:
Kapitel 5 Die Gesamtproteinmenge wu
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Kapitel 5 steigt mit steigendem pro
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Kapitel 5 Piano, A., Valbonesi, P.,
Page 141 and 142:
Publikationsliste • Marschner, L.
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