Laboratory Product Catalogue Total Laboratory Science Support

Centrisart ® Centrifugal Units for Rapid Sample Preparation
Ultrafiltration and Cell Harvesting Filtration
Ultrafiltrate
Ultrafilter
Sample
Centrifugal
force
Direction of
ultrafiltration
Particles etc.
Centrisart ® I for preparation of
protein-free ultrafiltrates.
Ready-to-use units for protein binding
studies and for removal of proteins from
biological samples, e.g. for determination
of creatinine in human serum.
Centrisart I consists of a sample tube and
a free-sliding inner tube with an ultrafilter
bottom. The principle of construction
turns the traditional method upside down:
ultrafiltration takes place opposite to the
direction of the centrifugal force.
This prevents blocking of the ultrafilter and
even allows filtration of particle-containing
samples. The ultrafiltrate is collected in the
inner tube and can be easily removed.
Specifications for Centrisart I
Dimensions
Of the Centrifugal tube, max. outer diameter, 14 mm.
93 mm long. Can be used in each laboratory centrifuge for 14 mm
or 17 mm+100 mm tubes.
Filtration area 0.79 cm 2
Materials
Max. centrifugal force
Polystyrene centrifugal tubes. Cellulose proprionate inner tube.
Cellulose triacetate or polysulfone ultrafilter. Polyethylene cap.
2,500+g (swing bucket rotor) and
2,000+g (fixed angle rotor)
Sample volume Max. 2.5 ml. Min. final volume, 100 µl
Ultrafiltration rate
Depends on the protein content of the sample, the temperature,
and on the ultrafilter cut-off used. In general, 2.5 ml sample volumes
can be ultrafiltered in 30 to 90 minutes.
Order numbers for Centrisart I
13209 E Trial pack, with 3 each units of 5,000, 10,000, 20,000 and
100,000 MWCO
13229 E With 5,000 MWCO cellulose triacetate ultrafilter, pack of 12
13239 E With 10,000 MWCO cellulose triacetate ultrafilter, pack of 12
13249 E With 20,000 MWCO cellulose triacetate ultrafilter, pack of 12
13269 E With 100,000 MWCO polyethersulfone ultrafilter, pack of 12
13279 E With 300,000 MWCO polyethersulfone ultrafilter, pack of 12
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