Laboratory Product Catalogue Total Laboratory Science Support

Technical data Sartobind MA affinity units
Protein Purification by Membrane Adsorbers Membrane
Chromatography
Data common for all membrane types
Membrane material
Stabilized reinforced cellulose
Number of layers 15
Bed height
4.0 mm
Bed volume
2.1 ml
Membrane diameter
25 mm
Adsorption area 75 cm 2
Dead volume
1.3 ml
Maximum pressure
0.6 MPa (6 bar, 87 psi)
Housing material
Polysulfone
Inlet connector
Female luer lock
Outlet connector
Male luer lock
Chemical stability
Stable in all common chromatography buffers except
peroxide and other oxidizing or reactive reagents
Data according to affinity type
Membrane type Protein A 75 IDA 75 Epoxy 75
Ligand Recombinant Iminodiacetic Epoxy
protein A acid (IDA)
Binding capacity 6 mg/unit 7.5 mg/unit 2–11 mg/unit
Test proteins polyclonal His 6 -tagged proteins**
human IgG protein*
depending on
sample
Recommended ions – Ni 2+ , Co 2+ , –
for coupling Cu 2+ or Zn 2+
Flow rate at 0.1 MPa > 10 ml/min > 10 ml/min > 10 ml/min
(1 bar, 14.5 psi)
Recommended flow rate 5–10 ml/min 50 ml/min 5–10 ml/min
pH stability (long term) 3–9 2–12 2–12
pH stability (short term) 2–10 1–14 1–14
Storage before use + 4°C Dry at room Dry at room
temperature temperature
* protected by patents of third parties
** proteins ranging from 12.5 to 600 kD under standard conditions
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