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Laboratory Product Catalogue Total Laboratory Science Support

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SuperSpinner – Disposable Membrane Aeration<br />

SuperSpinner S – Suspension Cultivation<br />

Spinners Cell<br />

Cultivation<br />

Systems<br />

The SuperSpinner is the next generation of<br />

incubator-based cell cultivation devices.<br />

Advanced technology, ease of operation and<br />

low cost make the SuperSpinner ideal for cell<br />

culture experiments. University, hospital and<br />

biopharmaceutical research laboratories can<br />

use the SuperSpinner for the production of<br />

biologicals, including monoclonal antibodies.<br />

Sartorius BBI Systems (BBI) has developed a<br />

new polycarbonate membrane holder with<br />

an integrated magnet.<br />

Functional principles and application<br />

The SuperSpinner is equipped with a<br />

patented bubble-free aeration system that<br />

also functions as a stirrer. This aeration<br />

technology has been proven for several<br />

years in various stirred tank bioreactors.<br />

The increased efficiency and improved<br />

productivity make the SuperSpinner the<br />

logical choice for replacing roller bottles<br />

and spinner flasks as well as mouse ascites<br />

techniques.<br />

Configuration of the SuperSpinner S<br />

The SuperSpinner consists of a 1 liter glass<br />

bottle. In addition, a setup with a 0,5 L<br />

glass bottle is also available. The center<br />

port contains the membrane aeration<br />

stirrer (MAS) and additional ports for feed,<br />

sampling and|or harvest connections.<br />

The SuperSpinner mounts on a magnetic<br />

drive platform.<br />

The membrane aeration stirrer is the highlight<br />

of the system. It consists of a frame wrapped<br />

with polypropylene (OXYPHAN ® ) tubing.<br />

Gas is pumped through the tubing via an air<br />

pump. Exhaust gas passes through a wash<br />

bottle. Temperature, dissolved oxygen and<br />

pH in the SuperSpinner are controlled by the<br />

incubator´s controlled environment.<br />

Mode of operation<br />

The complete setup is sterilized in an autoclave.<br />

Once cooled, the SuperSpinner is filled<br />

with medium. If necessary, this operation<br />

may be performed outside of a laminar flow<br />

hood using one of the ports in the center<br />

of the SuperSpinner and a pair of optional<br />

sterile connectors (female connector,<br />

Part no. BB-08809410, male connector,<br />

Part no. BB-08809402).<br />

Inoculation can be conducted in the same<br />

manner using the same sterile connectors.<br />

The SuperSpinner is then placed in an incubator<br />

on a magnetic drive platform. Aeration<br />

is initiated by switching on the air pump.<br />

Benefits of the new SuperSpinner<br />

disposable membrane aeration<br />

– Tested and proven<br />

– Easy to change<br />

– Disposable<br />

– No cleaning of aeration system necessary<br />

– Constant quality<br />

– FDA certificate included<br />

Material<br />

10 m (segmented) aeration membrane made<br />

of Polypropylene, hydrophobic, d a 380 µm,<br />

d i 280 µm, pore size 0.2 µm<br />

Ordering information SuperSpinner S<br />

8824503 SuperSpinner S 500<br />

8824562 Membrane aeration system S 05 disposable<br />

8824511 SuperSpinner S 1000<br />

8824843 Membrane aeration system S 10 disposable<br />

8810079 Air inlet|Exhaust filter, Midisart ® 2000<br />

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