Laboratory Product Catalogue Total Laboratory Science Support
Laboratory Product Catalogue Total Laboratory Science Support
Laboratory Product Catalogue Total Laboratory Science Support
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SuperSpinner – Disposable Membrane Aeration Combined with<br />
Microcarrier Application SuperSpinner C<br />
The SuperSpinner C is the next step into<br />
microcarrier application. This technology<br />
makes the SuperSpinner ideal for experiments<br />
with microcarriers. Various types of microcarriers<br />
can be used. Sartorius BBI Systems<br />
(BBI) has developed a new polycarbonate<br />
membrane holder with an integrated magnet<br />
and an insert for microcarriers. The aeration<br />
membrane is wound around the insert for<br />
microcarriers. The holder for the microcarriers<br />
is made of polyester, the mesh size is 100 µm.<br />
The insert can be filled with various types of<br />
microcarriers which are applicable and commercially<br />
available.<br />
Functional principles and application<br />
The mode of operation of the SuperSpinner C<br />
is similar to the operation of the Super-<br />
Spinner S. Once the microcarriers are filled<br />
into the module, they can be inoculated with<br />
surface dependent cells. After a period of<br />
gentle mixing, cells attach to the microcarriers<br />
and grow under very smooth conditions<br />
protected in the module. The polyester mesh,<br />
which separates the cells attached to the<br />
microcarriers and the medium, is 100% permeable<br />
for nutrients. Cell derived products,<br />
free-suspended cells, oxygen and carbon<br />
dioxide can easily pass the polyester mesh.<br />
Due to the tumbling movement of the<br />
insert, cells are sufficiently supplied with all<br />
necessary medium components and oxygen.<br />
This technology has been proven for several<br />
years in various types of fixed bed and<br />
fluid-bed bed bioreactors.<br />
Configuration of the SuperSpinner C<br />
The SuperSpinner C consists of a 1 liter glass<br />
bottle. The center port contains the insert for<br />
microcarriers, which is horizontally wound<br />
with aeration tubing. Additional ports for<br />
feed, sampling and|or harvest connections<br />
are also included. The SuperSpinner mounts<br />
on a magnetic drive platform.<br />
Mode of operation<br />
The bottom plate of the microcarrier insert<br />
is fixed by a screw and can be dismantled.<br />
The bottom plate can be removed and the<br />
microcarrier suspension can be filled into<br />
the chamber.<br />
The complete setup is sterilized in an autoclave.<br />
Once cooled, the SuperSpinner is filled<br />
with medium. If necessary, this operation<br />
may be performed outside of a laminar flow<br />
hood using one of the ports in the center<br />
of the SuperSpinner and a pair of optional<br />
sterile connectors (female connector,<br />
Part no. BB-08809410, male connector,<br />
Part no. BB-08809402).<br />
Inoculation can be conducted in the same<br />
manner using the same sterile connectors.<br />
The SuperSpinner is then placed in an incubator<br />
on a magnetic drive platform. Aeration<br />
is initiated by switching on the air pump.<br />
Spinners Cell<br />
Cultivation<br />
Systems<br />
Ordering information SuperSpinner C<br />
8824570 SuperSpinner C 1000<br />
8824546 Membrane aeration system C 10 disposable<br />
8810079 Air inlet|Exhaust filter, Midisart ® 2000<br />
Additional equipment for the SuperSpinner S and C<br />
8824720 Membrane pump for aeration<br />
8824827 Magnetic drive for SuperSpinner<br />
8809410 STT-Connector half, female 2 mm<br />
8809402 STT-Connector half, male 2 mm<br />
8809267 Slot membranes for STT connectors<br />
39971082 Silicone tubing 1.6+1.6 mm<br />
For operation under cGMP-conditions<br />
8824703 Magnetic drive with remote control<br />
8824711 Remote control Biomodule 40 B<br />
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