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Laboratory Product Catalogue Total Laboratory Science Support

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The Vivapure Range of Membrane Adsorber-Based Spin Columns<br />

for Rapid Protein Separation and Purification<br />

Spin Columns and Membrane Adsorbers Membrane<br />

Chromatography<br />

Vivapure Anti-HSA Kit<br />

Vivapure spin columns are available with<br />

a variety of different membrane adsorber<br />

chemistries.<br />

Vivapure ion exchange spin columns come<br />

in either strong or weak cation or anion<br />

charged membrane matrices. With these ion<br />

exchange membrane devices, protein binding,<br />

elution, and concentration is made almost<br />

as simple as filtration.<br />

There are two different sizes and<br />

capacities of Vivapure devices:<br />

A. Vivapure Maxi – 19/20 ml<br />

Binding capacities:<br />

High – H (60-80 mg)<br />

Medium – M (15-20 mg)<br />

B. Vivapure Mini – 400/500 ml<br />

Binding capacities:<br />

High – H (4 mg)<br />

Medium – M (1 mg)<br />

Low – L (0.02 mg)<br />

Vivapure membrane adsorbers are also<br />

available as Vivawell 96-well and Vivawell<br />

8-strip devices for increased throughput<br />

and robotic applications.<br />

Vivapure Anti-HSA Kit for Human<br />

Albumin Depletion<br />

Kit including 5 ml Anti-HSA affinity resin,<br />

clarification spin columns and buffers.<br />

The Vivapure Anti-HSA Kit utilizes unique<br />

antibody fragments for specific, fast and<br />

reproducible depletion of highly abundant<br />

human albumin from serum and plasma<br />

samples. The resolution of 2D-PAGE is<br />

significantly improved by this simple,<br />

20-minute, spin column-based protocol.<br />

Order No.: VS-SP08HAR<br />

Literature: SLU2011-e; SLU1506-e<br />

Vivapure Anti-HSA Affinity resin for Human<br />

Albumin Depletion<br />

50 ml Anti-HSA affinity resin utilizing unique<br />

antibody fragments for specific, fast and<br />

reproducible depletion of high abundant<br />

human albumin from serum and plasma samples.<br />

The resin can flexibly be filled in columns<br />

or spin columns. The resolution of 2D-PAGE is<br />

significantly improved by the simple, 20<br />

minute protocol.<br />

Order No.: VS-SP50HAR<br />

Literature: SLU1505-e; SLU-1506-e<br />

Vivapure C18 Micro spin columns<br />

Including 24 spin columns for preparation<br />

of samples prior to mass spectrometry, e.g.<br />

MALDI, MALDI-TOF and ESI. All steps are<br />

performed in the centrifuge offering high<br />

sample volume capacity (200 µl) and<br />

minimal hands-on time.<br />

Order no.: VS-RP218L24<br />

Literature: SL2020-e; SL-1042-e<br />

Vivapure AdenoPACK for Adenovirus<br />

Purification<br />

The AdenoPACK adenovirus purification and<br />

concentration kits offer researchers who need<br />

to recover up to 3+10 13 purified recombinant<br />

adenovirus particles for in-vitro transfection<br />

a fast, safe and easy to use solution. The kits<br />

include all reagents and devices necessary for<br />

clarification, purification and concentration of<br />

adenovirus type 5 from HEK293 cell cultures<br />

in only two hours. These straight forward kits<br />

replace time-consuming and labor-intensive<br />

48 hour CsCl density gradients.<br />

These kits are offered in 4 different versions<br />

with three different volume and virus<br />

purification capacities.<br />

Vivapure C18 Micro spin columns<br />

Vivapure Anti-HSA/IgG Kit for Human<br />

Albumin and IgG Depletion<br />

Kit including 5.5 ml Anti-HSA/IgG affinity<br />

resin, clarification spin columns and buffers.<br />

The Vivapure Anti-HSA/IgG resin utilizes<br />

unique antibody fragments and protein G for<br />

specific, fast and reproducible depletion of<br />

high abundant human albumin and IgG from<br />

serum and plasma samples. The resolution of<br />

2D-PAGE is significantly improved by the simple,<br />

20 minute, spin column-based protocol.<br />

Order No.: VS-SP08HAIGG<br />

Literature: SLU2013-e; SLU-1506-e<br />

Vivapure AdenoPACK 20<br />

Vivapure AdenoPACK 20 is the downscale<br />

kit in the AdenoPACK series, purifying up to<br />

1+10 12 viral particles from 20 ml cell culture.<br />

Especially when testing new constructs,<br />

parallel and fast purifications of different<br />

adenoviruses are essential. This kit allows<br />

the rapid, simple and affordable spin column<br />

based purification of 6 different samples in<br />

parallel and bridges a gap in the CsCl density<br />

gradient method – for the first time adenovirus<br />

can efficiently be purified from less<br />

than 100 ml cell culture volume!<br />

Order No.: VS-AVPQ020<br />

Literature: SL-6512-e; SLU-1518-e<br />

126

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