Laboratory Product Catalogue Total Laboratory Science Support

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Technical data Sartobind MA affinity units Protein Purification by Membrane Adsorbers Membrane Chromatography Data common for all membrane types Membrane material Stabilized reinforced cellulose Number of layers 15 Bed height 4.0 mm Bed volume 2.1 ml Membrane diameter 25 mm Adsorption area 75 cm 2 Dead volume 1.3 ml Maximum pressure 0.6 MPa (6 bar, 87 psi) Housing material Polysulfone Inlet connector Female luer lock Outlet connector Male luer lock Chemical stability Stable in all common chromatography buffers except peroxide and other oxidizing or reactive reagents Data according to affinity type Membrane type Protein A 75 IDA 75 Epoxy 75 Ligand Recombinant Iminodiacetic Epoxy protein A acid (IDA) Binding capacity 6 mg/unit 7.5 mg/unit 2–11 mg/unit Test proteins polyclonal His 6 -tagged proteins** human IgG protein* depending on sample Recommended ions – Ni 2+ , Co 2+ , – for coupling Cu 2+ or Zn 2+ Flow rate at 0.1 MPa > 10 ml/min > 10 ml/min > 10 ml/min (1 bar, 14.5 psi) Recommended flow rate 5–10 ml/min 50 ml/min 5–10 ml/min pH stability (long term) 3–9 2–12 2–12 pH stability (short term) 2–10 1–14 1–14 Storage before use + 4°C Dry at room Dry at room temperature temperature * protected by patents of third parties ** proteins ranging from 12.5 to 600 kD under standard conditions 132
<strong>Product</strong> and ordering information Sartobind Description Pore size Capacity Sartobind Sartobind Sartobind Sartobind Sartobind Sartobind Membrane (µm) (µg/cm 2 )* MA 5 MA 15 MA 75 MA 100 SingleSep System S Strong acidic cation >3 800 • • • • • • exchanger sulfonic acid Q Strong basic anion >3 800 • • • • • • exchanger quaternary ammonium C Weak acidic cation >3 600 • • • • • exchanger carboxylic acid D Weak basic anion >3 600 • • • • (•) exchanger diethylamine Protein A Recombinant 0.45 80 • (•) protein A affinity IDA Iminodiacetic acid >3 100 • (•) metal chelate Epoxy Epoxy-activated 0.45 30 • (•) membrane * Minimum static capacity for standard or target protein, • available; (•) available as special product Protein Purification by Membrane Adsorbers Membrane Chromatography Order No. Description Quantity S5F Sartobind S 5 strong cation exchanger 15 Q5F Sartobind Q 5 strong anion exchanger 15 C5F Sartobind C 5 weak cation exchanger 15 D5F Sartobind D 5 weak anion exchanger 15 S15X Sartobind S 15 strong cation exchanger 2 Q15X Sartobind Q 15 strong anion exchanger 2 C15X Sartobind C 15 weak cation exchanger 2 D15X Sartobind D 15 weak anion exchanger 2 Q75X Sartobind Q 75, strong anion exchanger 2 S75X Sartobind S 75, strong cation exchanger 2 C75X Sartobind C 75, weak cation exchanger 2 D75X Sartobind D 75, weak anion exchanger 2 S100X Sartobind S 100 strong cation exchanger 1 Q100X Sartobind Q 100 strong anion exchanger 1 C100X Sartobind C 100 weak cation exchanger 1 D100X Sartobind D 100 weak anion exchanger 1 93PR-A06DB-12--V Sartobind Protein A 75, recombinant protein A 2 93IDA-42DB-12--V Sartobind IDA 75, iminodiacetic acid 2 93EPOX06DB-12--V Sartobind Epoxy 75, epoxy activated 2 17002---140 Pair of Luer Lock adapters black Tefzel 2 to connect MA 5, 15, 75 and 100 to a liquid chromatography system 133
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Laboratory Product Catalogue Total
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About Sartorius Sartorius AG is an
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Filtration Applications Major Filtr
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Concentration of Proteins The Sarto
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Comparison of the adsorption of var
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Other water testings 142 mm diamete
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Filtration Filtration Membrane Filt
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Order numbers for cellulose acetate
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Polyethersulfone Membrane Filters,
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Order numbers for cellulose nitrate
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Hydrophobic PTFE Membrane Filters,
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Polycarbonate Track-Etch-Membrane F
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Ultrafiltration Membrane Filters fr
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Minisart ® 0.2 µm Syringe Filter
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Specifications for Minisarts, Minis
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Minisart-NY25 (Nylon) Versatile fil
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Order numbers for Minisart SRP4 178
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Polycarbonate holder for aqueous so
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Polycarbonate holder for aqueous so
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Accessories for Ready-to-Use Minisa
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25 mm Glass Holder for the Filtrati
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All-glass Holder for Particle Remov
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Order numbers for 47 mm polycarbona
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Water jet pump with G3/4 female thr
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Sartolab ® P20 and Sartolab ® P20
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SartoScale Filter Test Disposables
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Sartobran ® P 150 and Sartobran ®
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Type 00, with hose nipple inlet and
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Order numbers for Sartopore 150 Wit
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Mini Filter Cartridges for the Part
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Sartoclear ® P Depth Filter Capsul
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Low-Cost Polycarbonate Holder for t
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47 mm Stainless Steel Filter Holder
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142 mm in-line PTFE holder This fil
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Stainless Steel Holder with 2 Liter
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GMP-complying 142 mm Stainless Stee
Page 81 and 82: Modular Assembly System for Stainle
Page 83 and 84: Filtration Systems with Pressure Ta
Page 85 and 86: Order numbers 17530 5 liter capacit
Page 87 and 88: With commercially available hoses T
Page 89 and 90: Order numbers for PTFE pressure hos
Page 91 and 92: Order numbers for Midisart 2000 uni
Page 93 and 94: Sartofluor ® MidiCaps with PTFE Me
Page 95 and 96: Hydrophobic PTFE Membranes, Type 11
Page 97 and 98: 25 mm Stainless Steel Filter Holder
Page 99 and 100: Sartofluor ® Mini Cartridges for H
Page 101 and 102: Housings for Sterile Air Venting an
Page 103 and 104: Ordering information Vivaspin 2, 0.
Page 105 and 106: Ordering information Vivaspin 15, 2
Page 107 and 108: Vivapore Solvent Absorption Concent
Page 109 and 110: Ordering information Vivacell 70, 1
Page 111 and 112: Sartocon ® Slice The Pilot-Scale C
Page 113 and 114: Specifications Drive Motor 24 V DC
Page 115 and 116: Sartocon ® Slice 200 Stainless Ste
Page 117 and 118: Filter Papers Introduction Generall
Page 119 and 120: Chemical Compatibility 1. Filter Ma
Page 121 and 122: 2. Filter Holder, Cartridge Housing
Page 123 and 124: 3. Ready-to-Connect Filtration Unit
Page 125 and 126: Membrane Chromatography Membrane Ch
Page 127 and 128: Vivapure AdenePACK 100 Vivawell Met
Page 129 and 130: Sartobind ® The Pace Maker in Memb
Page 131: Technical data Sartobind MA ion exc
Page 135 and 136: Sartobind ® System For a complete
Page 137 and 138: Microbiological Analysis Air Monito
Page 139 and 140: AirPort MD8 Battery-Powered Portabl
Page 141 and 142: Specifications of gelatine filters
Page 143 and 144: Accessories for remote control func
Page 145 and 146: The membrane filters All membranes
Page 147 and 148: Microsart e.motion Membrane Filters
Page 149 and 150: White membrane with black grid, for
Page 151 and 152: White membrane with black grid, for
Page 153 and 154: Specifications Design Growth Promot
Page 155 and 156: Cellulose nitrate membrane filters,
Page 157 and 158: Order numbers for nutrient pad sets
Page 159 and 160: Order numbers for nutrient pad sets
Page 161 and 162: Lactose broth media, bottled concen
Page 163 and 164: Biosart ® 100 Monitors, 100 ml, 47
Page 165 and 166: Biosart ® 250 Funnels In microbiol
Page 167 and 168: Specifications Stainless steel qual
Page 169 and 170: Accessories and replacement parts f
Page 171 and 172: Specifications Stainless steel mult
Page 173 and 174: Individual stainless steel filter h
Page 175 and 176: Water traps Used between suction fl
Page 177 and 178: Order numbers traditional pumps Des
Page 179 and 180: Stainless steel tweezers Membrane f
Page 181 and 182: Contents Parts supplied Aluminium c
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Order no. for universal pump Descri
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Additional accessories for reusable
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Bags for Fluid Handling Sterile Flu
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Sterile Fluid Handling Bags: 5 Lite
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Laboratory Water Systems arium ® W
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Ambient temperature & humidity Oper
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RO system schematic Inlet Pressure
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Flow chart 5 µm Prefilter Outlet P
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Ambient temperature and humidity Op
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Sartorius TOC Instrument - Electron
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arium ® Pressure Tanks - Product W
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arium ® 613CPM2--------V - Reverse
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Cartridge kit for 611DI and 611UF s
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Cell Cultivation Systems Benchtop S
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CERTOMAT ® Incubation Shakers CERT
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Reference BBI-885 3134 BBI-885 3142
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Specifications Microbiological grad
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SuperSpinner - Disposable Membrane
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Ordering information Description BI
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Homogenizers and Centrifuges Mikro-
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Accessories for homogenizers Mikro-
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Filter Integrity Testing Systems Sa
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Specifications for Sartocheck Junio
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Technical specification Power requi
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Technical specifications Power requ
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Laboratory Equipment for Electroche
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Specifications pH Measurement Docu-
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Specifications pH measurement PP-15
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Sensors for the Highest Quality Mea
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Accessories Order number Printer fo
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Weighing Equipment for the Laborato
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Specifications Model SE2*** ME5***
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Specifications Model ME235S ME235P
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Specifications Model Read- Weighing
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Specifications and accessories Mode
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Accessories for ME and SE2 Models O
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Accessories for LE, CP and ED Model
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Specifications Model Weighing capac
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Which density kit for which balance
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Equipment for Neutralizing Static E
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Specifications Model Maximum Readab
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Weight Sets (YCS) Features of Sarto
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Metrological Weights (YCW) Class E1
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Accessories for Weights (YAW) Acces
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Specifications Capacity Readability
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Moisture and Water Analysis Moistur
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Specifications MA35|MA100|MA150 MA3
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Accessories MA35|MA100| MA150 Acces
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Technical Specifications|Accessorie
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Specifications* Measuring range (%)
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FACTS ® Service Progam Added Value
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30 days commitment CONFIDENCE ® -
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Training Examples for the Pharmaceu
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Training Examples for the F & B Ind
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America Asia|Pacific USA Sartorius
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Application|Product name Page Appli
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Index by Order Numbers Order no. Pa
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Order no. Page Order no. Page Order
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Laboratory Product Catalogue Total Laboratory Science Support

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