Laboratory Product Catalogue Total Laboratory Science Support
Laboratory Product Catalogue Total Laboratory Science Support
Laboratory Product Catalogue Total Laboratory Science Support
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Sartobind ® SingleSep<br />
Protein Purification by Membrane Adsorbers Membrane<br />
Chromatography<br />
Sartobind SingleSep ion exchange capsules<br />
are designed to remove contaminants at<br />
accelerated flow rates. This is a direct result<br />
of negligible mass transfer effects and is<br />
made possible by the >3 µm macroporous<br />
membrane. The design allows for robust<br />
chromatographic separations and drastically<br />
reduced validation costs. SingleSep capsules<br />
are used for DNA removal from therapeutic<br />
proteins, host cell protein removal and viral<br />
clearance.<br />
Technical data Sartobind SingleSep capsules<br />
– Ready-to-use format<br />
– Simple and fast set-up<br />
– No trouble with air entrapment,<br />
channeling or bed cracking<br />
– Membrane pore size of > 3 µm allows<br />
purification of large biomolecules and<br />
viruses<br />
– Low unspecific adsorption = less<br />
product loss<br />
– Reduced validation costs<br />
– Autoclaving at 121°C for 30 min,<br />
one cycle<br />
Membrane Base material Stabilized reinforced cellulose<br />
Membrane thickness 275 µm<br />
Membranes<br />
– strong cation exchanger S (sulfonic acid)<br />
- strong anion exchanger Q<br />
(quaternary amine)<br />
Capsule Design cylindric, nominal number of layer: 15<br />
Bed height<br />
4 mm<br />
Material Capsule Polypropylene<br />
Operation Max. pressure O.4 MPa (4 bar|58 psi)<br />
Technical Data<br />
Order number Description Connector Nominal Bed Quantity<br />
capsules protein volume<br />
binding [ml]<br />
capacity<br />
[g]<br />
92IEXQ42D4-OO—A Sartobind Q SingleSep Hose barb 0.2 7 4<br />
mini capsule<br />
92IEXS42D4-OO—A Sartobind S SingleSep Hose barb 0.2 7 4<br />
mini capsule<br />
92IEXQ42D4-SS—A Sartobind Q SingleSep Sanitary 0.2 7 4<br />
mini capsule<br />
92IEXS42D4-SS—A Sartobind S SingleSep Sanitary 0.2 7 4<br />
mini capsules<br />
92IEXQ42D9-OO—A Sartobind Q SingleSep Hose barb 2 70 4<br />
5“ capsules<br />
92IEXS42D9-OO—A Sartobind S SingleSep Hose barb 2 70 4<br />
5“ capsules<br />
92IEXQ42D9-SS—A Sartobind Q SingleSep Sanitary 2 70 4<br />
5“ capsule<br />
92IEXS42D9-SS—A Sartobind S SingleSep Sanitary 2 70 4<br />
5“ capsules<br />
Minimum static binding capacity was measured with bovine serum albumin and hen egg<br />
lysozyme: 0.8 mg/cm 2 for Q and S membranes.<br />
For further scale-up, please request the Sartobind SingleSep brochure. Order no. SL-1521-e.<br />
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