Laboratory Product Catalogue Total Laboratory Science Support

Biotechnology Workshop
# 862023
High cell density cultivation (HCDC) of
Escherichia coli and primary downstream
processing (DSP)
Emphasis is placed in Part I on an efficient
high cell density cultivation (HCDC), on the
safe handling of bench-scale bioreactors, as
well as on the avoidance of sterility problems.
Consideration is given to both a sufficient
oxygen supply and fundamentals of the
genetics of Escherichia coli and suitable
vectors. Part II provides an introduction to
primary downstream processing techniques,
e.g. primary steps to isolate the protein of
interest, so-called product capturing.
Contents
– Bench-scale bioreactors: handling from
preparation to cell harvest
– Sterility techniques, sterilization kinetics
– Composition of media for HCDCs
– Strategies for cultivation and analysis
– Escherichia coli and vectors
– Primary downstream processing
– Centrifugation
– Crossflow filtration (microfiltration
and ultrafiltration)
– Cell disruption methods
– Expanded bed adsorption
– Qualification & validation
Target group
Attendees include biologists, biochemists,
chemists, biotechnologists, engineers and
technical assistants with basic knowledge of
microbiology, cultivation of microorganisms
and of molecular biology, and who are in
charge of managing bioreactors or the downstream
processing of bioprocess products or
willing to do this in future.
# 862008
The use of crossflow filtration in the
pharmaceutial and biotech industry
The participants will acquire state-of-the-art
knowledge about GMP-compliant processing
using crossflow filtration.
Theoretical section
– Crossflow filtration theory
– Membrane characterization|membrane
selection
– Factors critical to performance
– Scaling up
– Operating conditions
– Cleaning-in-place (CIP)
– Steaming-in-place (SIP)
– Integrity testing
– Applications in biotechnology
Practical section
– Operational set-up of the systems
– Determination of the flux rate for water
– Demonstration of steaming in place
(optional)
– Cell (particle) retention by microfiltration
(model solution)
– Concentration of protein solutions
by ultrafiltration
– Removal of low-molecular weight
constituents by diafiltration
– Cleaning
– Discussion of the results
Total course time
2 days
Target group
Staff working in areas ranging from research
to production. The modular structure of the
course allows the participants to request their
own topics as well.
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