School of Engineering and Science - Jacobs University
School of Engineering and Science - Jacobs University
School of Engineering and Science - Jacobs University
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Material <strong>and</strong> Methods<br />
Tab. 4. Antibiotic substances used in these studies.<br />
Antibiotic<br />
Stock solution<br />
concentration<br />
End<br />
concentration in<br />
medium<br />
Ampicillin (Amp r ) 50 mg/ml 50 mg/L<br />
Chloramphenicol (Cm r ) 25 mg/ml 25 mg/L<br />
Kanamycin (Km r ) 25 mg/ml 25 mg/L<br />
Spectinomycin (Sp r ) 25 mg/ml 25 mg/L<br />
3.3 Kits<br />
Tab. 5. Kits used in these studies.<br />
Kit<br />
DIG DNA Labeling <strong>and</strong> Detection Kit<br />
Taq core Kit<br />
pGEM-Teasy Vector system I<br />
NucleoSpin Plasmid<br />
QIAquick Gel extraction kit<br />
QIAquick PCR Purification kit<br />
Manufacturer<br />
Roche (Mannheim)<br />
Qiagen (Hilden)<br />
Promega (Mannheim)<br />
Macherey-Nagel (Düren)<br />
Qiagen (Hilden)<br />
Qiagen (Hilden)<br />
3.4 Media<br />
All media were sterilized in an autoclave (30 min, 121°C, 1.3 bar). Some<br />
components <strong>of</strong> the media were sterilized by filtration through a sterile filter <strong>of</strong><br />
0.2 µm pore size (Whatman). 1.5 % agar was added to a medium used for<br />
bacterial growth on a plate.<br />
20
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