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Material and Methods Tab. 6 continued. Bacterial strains used in these studies. Bacterial strain Relevant characteristics Reference or source Pantoea agglomerans 48b epiphyt, DFFE producer Völksch et al. , 1996 Dickeya chrysanthemi cbsE-1 tonB60 Xanthomonas campestris DSM 1050 cbsE-1 , tonB60 , Cbs – , TonB – , Spec R , Km R Enard and Expert 2000 Ach - , but contains homologue siderophore synthethase German Culture Collection (DSM) Sinorhizobium meliloti DSM 6047 Produces rhizobactin German Culture Collection (DSM) Pseudomonas syringae pv. syringae 22d/93 (Pss22d) antagonist wild type; Pvd + , Ach + Völksch et al. , 1996 Pss22d∆Pvd Pss22d∆Ach Pss22d∆Sid derivative of Pss22d; Pvd - , Ach + , Km r derivative of Pss22d; Pvd + , Ach - , Km r derivative of Pss22d∆Pvd; Pvd - , Ach - , Km r , Sp r this study this study this study glycinea 1a/96 pathogen wild type; Pvd+, Ach+ Völksch and May, 2002 glycinea PG4180 Bender et al. , 1993 aceris CFBP 2339 actinidiae MAFF 302091 aptata GSPB 1080 atrofaciens GSPB 1440 atropurporea MAFF 301309 berberis CFBP 1727 broussonetiae MAFF 810036 J-M Meyer, Strasbourg, France David Guttman, Toronto, Canada Helge Weingart / K. Rudolph German Culture Collection (DSM) B. Völksch , Jena J-M Meyer, Strasbourg, France David Guttman, Toronto, Canada castaneae CFBP 4217 J-M Meyer, Strasbourg, France coronafaciens DSM 50261 strains tested for the Helge Weingart, Bremen daphniphylli CFBP 4219 achromobactin-like J-M Meyer, Strasbourg, France siderophore delfinii ICMP 529 Kawalleck et al. (1995) mori MAFF 301020 David Guttman, Toronto, Canada mosprunorum GSPB 1013 K. Rudolph, Göttingen maculicola DC3000 papulans ICMP 4048 phaseolicola 1448A Elina Roine, Helsinki, Finnland John Lydon, Beltsville, USA John W. Mansfield pisi GSPB 1477 K. Rudolph, Göttingen savastanoi GSBP 2259 K. Rudolph, Göttingen tabaci GSPB 117 K. Rudolph, Göttingen tomato 487 B. Völksch , Jena 25
Material and Methods 3.6 Plasmids Tab. 7. Plasmids used in these studies. Plasmid Relevant characteristics Reference/Source pGEM-TEasy cloning vector for T/A cloning, Amp r Promega pPVSA1 pPVSA2 1,4 kb PCR-product of pvsA (fragment1) in pGEM-T Easy, primer combination pvsA_fwd1_Spe and pvsA_rev6_Kpn , template Pss22d, Amp r 1,6 kb PCR-product of pvsA (fragment2) pGEM-T Easy, primer combination pvsA_fwd7_Kpn und pvsA_rev4_Bam , template Pss22d, Amp r This study This study pPVSA3 Spe I-Kpn I fragment of pPVSA1 in pPVSA2 This study pPVSA4 pPVSA3 containing Km r -cassette (Kpn I-Fragment), suizidvector for pvsA-mutagenesis in Pss22d This study pACS1 1.3 kb PCR fragment amplified by primer combination Achr1_fwd and Achr2_Kpn I_rev. in pGEM-T Easy, Amp r This study pACS2 2 kb PCR fragment amplified by primer combination Achr5_EcoRV_fwd and Achr6_rev in pGEM-T Easy, Amp r This study pACS3 pACS1 with Km r resistance cassette, Amp r , Km r This study pACS4 pACS3 insert in pACS2, suizide vector for mutagenesis of acsgenes in Pss22d, Amp r , Km r This study pBBR-MCS1 cloning vector, Cm r Kovach et al., 1994 pBBR-11-Sid 6 kb Ps tI-fragment of Pss22d∆Sid in pBBR-MCS1, Cm r , Sp r This study pBBR-23-Sid 6 kb Ps tI-fragment of Pss22d∆Sid-23 in pBBR-MCS1, Cm r , Sp r This study pMKm vector containing kanamycin cassette, Km r Murillo et al., 1994 pCAM-Not Sp r Amp r , carries the mTn5 SS40 transposon Wilson et al., 1995, H. Weingart 26
Page 1 and 2: Siderophore production of Pseudomon
Page 3 and 4: Acknowledgements This project was s
Page 5 and 6: Abbreviations Ach achromobactin-lik
Page 7 and 8: Contents 4.1.2 Pseudomonas syringae
Page 9 and 10: Contents 5.3 Influence of sideropho
Page 11 and 12: Introduction 2 Introduction Like hu
Page 13 and 14: Introduction In contrast to the rhi
Page 15 and 16: Introduction 2.1.2 Host, pathogen a
Page 17 and 18: Introduction The relationship betwe
Page 19 and 20: Introduction developement of resist
Page 21 and 22: Introduction Fe 2+ + H 2 O 2 → Fe
Page 23 and 24: Introduction 1997a; May et al., 199
Page 25 and 26: Introduction Pss22d demonstrated a
Page 27 and 28: Introduction 2.4 Aim of this study
Page 29 and 30: Material and Methods Tab. 4. Antibi
Page 31 and 32: Material and Methods Iron-free 5b m
Page 33: Material and Methods Solution3, car
Page 37 and 38: Material and Methods PCR-Screening
Page 39 and 40: Material and Methods on ice for 10
Page 41 and 42: Material and Methods stained in eth
Page 43 and 44: Material and Methods 4.2.5.3 Conver
Page 45 and 46: Material and Methods 4.2.5.9 Ligati
Page 47 and 48: Material and Methods strains were s
Page 49 and 50: Material and Methods 9.5). For sign
Page 51 and 52: Material and Methods gentle shaking
Page 53 and 54: Material and Methods columns were w
Page 55 and 56: Results 5 Results 5.1 Analysis of s
Page 57 and 58: Results The 13-kb open reading fram
Page 59 and 60: Results If integration is due to a
Page 61 and 62: Results Yersiniabactin is a siderop
Page 63 and 64: Results Next, a large number of tra
Page 65 and 66: Results Nevertheless the site of tr
Page 67 and 68: Results Achr2_KpnI_rev binding site
Page 69 and 70: Results Interestingly the mutant Ps
Page 71 and 72: Results XAD4-purification yielded i
Page 73 and 74: Results After changing the mobile p
Page 75 and 76: Results P P A Fig. 21. Isoelectic f
Page 77 and 78: Results reduced in comparison to th
Page 79 and 80: Results A-E represents single inocu
Page 81 and 82: Results Tab. 10. Development of pop
Page 83 and 84: Results Tab. 11. Distribution of th
Page 85 and 86:
Discussion bacteria with redundande
Page 87 and 88:
Discussion 6.2 Possible regulation
Page 89 and 90:
Discussion stationary phase transit
Page 91 and 92:
Discussion and bacterium (Loper et
Page 93 and 94:
Literature Bultreys, A., Gheysen, I
Page 95 and 96:
Literature Galperin, M. Y. (2006).
Page 97 and 98:
Literature Mazzola, M. (2002). Mech
Page 99 and 100:
Literature Schubert, S., Rakin, A.,
Page 101:
Literature Zou, J., Rodriguez-Zas,
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